Fred Liers PhD restore liquid mineral supplement gut healthHeard of RESTORE liquid mineral supplement? If not, you’re about to discover something important for gut health.

RESTORE™ is formulated to help restore gut health. It works by strengthening the integrity of tight junctions in your intestinal wall.

Maintaining tight junctions is important because they protect your body from substances entering the blood stream. Restore also supports the communications systems of the bacteria in your microbiome.

Tight junctions are natural barriers protecting the lining of your gut barrier, organs such as kidneys and heart, and the blood-brain barrier thereby keeping out unwanted substances. Even as we take in nutrients from the GI tract, the function of tight junctions is maintaining the integrity of the gut wall and other barriers that protect the body.

These days, our gut membrane has a tough job keeping out unwanted substances. Because tight junctions are weakened by many things, including highly processed foods, especially those containing gluten and gliadin, the herbicide glyphosate (the primary ingredient in RoundUp weedkiller), and other exposures like electromagnetic fields (EMF) from digital devices and Wi-Fi networks.

As a result of weakened tight junctions, undesirable substances from food proteins, harmful chemicals and other toxins can slip though into our bloodstream. This can lead to a powerful immune response that can weaken immunity and cause significant inflammation. Foreign substances in the bloodstream can also result in extreme food allergies and food sensitivities to various toxins entering the body.

We live in an age in which our microbiome health is under assault. Studies show our ancient ancestors had 20,000–30,000 different strains of beneficial bacteria in their GI tracts. Many people today have just 5,000 or less. Why is that?

There are many reasons for low microbiome diversity. These reasons include antiobiotic use (both from pharmaceuticals and foods), glyphosate (which essentially acts like an antibiotic), processed foods (especially those containing gluten and gliadin), and systemic degradation of the gut environment rendering it less hospitable for maintaining health populations of gut flora.

These impacts are significant because diversity in your microbiome not only supports gut health, but also appropriate brain health, immune system health, and overall vitality. Restore acts as a “microbiome fertilizer” helping establish and maintain healthy microbiota.

In fact, Restore contains important “redox” molecules produced millions of years ago by existing soil bacteria. These molecules are an important part of communications systems within the body between gut bacteria and with the body’s mitochondria. These communication systems play an important role in establishing proper homeostasis throughout the body.


There are important reasons to try Restore—whether or not you experience significant gut health issues. These reasons include:

• Maintaining strong tight junctions for mega membrane health, and helping the body restore tight junction integrity

• Creating an environment in which beneficial bacteria can thrive

• Boosting microbiome diversity

• Healing the gut

• Mitigating the adverse effects substances like antibiotics, gluten/gliadin, glyphosate, and GMOs on microbiome health

• Supporting immune system function

• Protecting integrity of blood-brain barrier

Other benefits include support for respiratory wellness, enhancing mental clarity, and promoting proper homeostasis throughout the body.

For all these reasons and more, you should consider taking Restore. You can start by taking one teaspoon or less daily, and gradually increasing your dose to one tablespoon or more.Restore for gut health liquid supplement

I have taken Restore daily for more than two years, and benefit greatly from it. The most noticeable effects are a complete cessation of spring allergies (after two decades of worsening symptoms), greater clarity of thinking, and improved overall well being. I have seen other people get good results with a variety of conditions from dysbiosis to gluten/gliadin sensitivity.


You can learn more about Restore on our website. Also, the Restore website offers in-depth information and videos. Here is how the Restore website and Dr. Zach Bush describe the formula:

Restore is a dietary supplement supplying first line defense against daily exposure to environmental and food-borne factors. It helps create an optimal environment or ecology in the gut membrane for beneficial or “good” bacteria to flourish. Beneficial gut flora protect your immune system and maintain health. Restore is a scientifically proven soil-derived mineral supplement designed to support protection of the intestinal walls.

Restore protects the entire gastrointestinal (GI) tract against agricultural herbicides, antibiotics, GMOs, gluten, and food-borne toxins. It helps create an environment in which 20,000–30,000 different strains of beneficial gut bacteria can thrive. This is a far different mode of action from probiotics, most of which contain just 3–30 strains of beneficial bacteria.

Restore helps create a strong immune system along with proper gut function. Microbiome balance and tight junction integrity are known to constitute a significant part of the immune system. They directly affect DNA transcription of cells to promote optimal health and prevent a leaky gut syndrome, thereby improving gut intelligence.

Seventy percent (70%) of our immune system is found in our gut. We need tens of thousands different strains of good gut bacteria to support a healthy immune system. Clinical experience, in addition to cell culture studies, support the theory that Restore’s proprietary formula creates the optimal environment in the gut to create a tight junction barrier and maintain our health and wellness.

Restore is a daily liquid supplement designed to support the protection of the entire gastrointestinal (GI) tract against agricultural herbicides, antibiotics, GMOs, gluten and food-borne toxins by helping to create an environment where between 20,000-30,000 different strains of good gut bacteria thrive. Most probiotics on the market contain fewer than 24 different strains of good gut bacteria.


Restore is ideal for individuals suffering from gut health issues. Yet, it is also excellent for supporting and maintaining gut health generally. That is the reason we at HPDI consider Restore important enough to designate as a foundational supplement, one of the top six supplements you need for good health.

Given the safety and effectiveness of Restore, there is broad applicability for its use. This is especially true because gut and microbiome health are under constant assault. These assaults range from multiple environmental and lifestyle factors—including poor diets high in processed foods, antibiotics in pharmaceuticals and animal foods—to massive glyphosate (herbicide) contamination of foods, soils, air, and water.

Let Restore be your first line of defense against assaults on your gut health, tight junctions, microbiome diversity, and immune function. As it says on the bottle, “Complete well-being begins in the gut.”


Gut Health – Effects of Glyphosate and Antibiotics
by Hank Liers, PhD and Fred Liers, PhD

Restore – 32 oz

Restore – 8 oz

HPDI Gut Health Formulas

HPDI Foundational Supplements

Restore blog (Restore4Life)



Fred Liers PhD hydrogen supplements drinks H2I love hydrogen supplements. So should you. Since starting my morning—and often afternoon—routine of making hydrogen drinks in the kitchen, I have come to appreciate the surge of energy lasting many hours, as well as a host of other benefits.

Most of all, I am amazed how easy hydrogen supplements are to use considering how much they do for you, and how well they complement the supplements I already take—seeming to make them all more effective.

I typically use one or two effervescing Active H2 Ultra tablets or Vital Reaction tablets in 8–16 ounces of water. Then I like to open one Megahydrate capsule directly in the effervescing water. I wait about 90 seconds to let the tablets stop “fizzing” and then I drink immediately. Allowing the drink to sit for a few minutes is okay, but the sooner you drink it, the higher the concentration of hydrogen.


Hydrogen supplements are among the newer, cutting-edge nutritional formulas available to support optimal health. More than 500 scientific articles now support the therapeutic potential hydrogen for essentially every organ system and in 150 human disease models, according to the non-profit Molecular Hydrogen Foundation. In fact, hydrogen is rapidly incorporated into medicine, sports medicine, peak performance, elite fitness, and more.

Hydrogen is an antioxidant. It is also an extremely small molecule that can penetrate even the tiniest cellular compartments. This helps explain how hydrogen works to offer free-radical defense throughout the entire body.

The medical and scientific literature is clear. Hydrogen’s modes of action:

  • H2 reduces oxidative stress as a selective antioxidant and by maintaining homeostatic levels of glutathione, superoxide dismutase, catalase, etc.
  • H2, like other gaseous signaling molecules (i.e. NO, CO, H2S), appears to have cell signal-modulating activity affording it with anti-inflammatory, anti-obesity, and anti-allergy benefits.

We include hydrogen supplements in HPDI’s system of Foundational Supplements. In fact, hydrogen formulas as just one of six foundational supplements. We consider hydrogen a “secondary” foundational supplement usually to be added to a supplement regimen after the four primary ones: multivitamins, antioxidant & vitamin C formulas, essential fatty acids, and superfoods high in dietary nucleic acids (i.e., Rejuvenate! superfoods).


The scientific literature discusses the use of molecular hydrogen for many clinical applications, including:

• Metabolic Syndrome including diabetes, hyperlipidemia, arteriosclerosis, hypertension, and obesity

• Ischemia/reperfusion injuries, including cerebral and myocardial infarctions, organ transplants, post-cardiac arrest

• Neuroprotection, including applications for dementia, Parkinson’s disease, depression, and anesthesia

• Inflammation, including applications for polymicrobial sepsis, rheumatoid arthritis, wound healing, and bowel diseases

• Mitochondrial diseases

• Hemodialysis and ventilation

• Aging, including cognitive decline

• Exercise, including applications for fatigue, lactic acid, recovery, and oxidative stress related to heavy exercise

Side effects of cancer therapies, including radiotherapy and chemotherapy

• Many other benefits

(Source: Molecular Hydrogen Foundation)


According to the Molecular Hydrogen Foundation, there are three ways molecular hydrogen exerts positive health effects.

1. Molecular hydrogen easily diffuses into subcellular compartments where it scavenges cytotoxic oxygen radicals, thereby protecting DNA, RNA, and proteins against oxidative stress.

2. Molecular hydrogen triggers activation or upregulation of additional antioxidant enzymes (e.g., glutathione, superoxide dismutase, catalase, and others) and/or cytoprotective proteins of the body.

3. Molecular hydrogen may be a novel signaling molecule that alters cell signaling, cell metabolism, and gene expression. This may explain its apparent anti-inflammatory, anti-allergic, and anti-apoptotic (or anti-cell death) effects.

hydrogen supplements Active H2 Ultra tablets

Active H2 Ultra hydrogen supplement (60 tablets).


Molecular hydrogen exhibits great safety, and it is regarded as safe for use in the body. It is shown no toxicity even in high concentrations.

Safety standards have long been established for high concentrations of hydrogen for inhalation largely because of the history of high-pressure H2 gas used in deep-water diving gas mixtures for preventing decompression sickness.

Notably, H2 gas combusts only at temperatures higher than 527 °C, and it explodes by chain reaction with oxygen (O2) only in the range of H2 concentration (4–75%, vol/vol).

Molecular hydrogen is used for medical applications safely by several ingestion methods including inhalation of 1–4% hydrogen gas, for example, which exhibits great effectiveness. All these factors mean that hydrogen is safe, easy-to-use, and effective for therapeutic purposes.

vital reaction hydrogen supplements

Vital Reaction hydrogen supplement (60 tablets).


According to the Molecular Hydrogen Foundation, drinking H2-rich water is the easiest, and often the most effective, method for obtaining hydrogen.

Drinking hydrogen-infused water is easy to do, and convenient as you can drink it at home or while traveling. Effective hydrogen products HPDI carries include Vital Reaction tablets, Active H2 tablets, and Megahydrate capsules.

As noted, I make one or two hydrogen drinks daily, first in the morning and then often again in the afternoon. I drink them on an empty stomach. I enjoy a significant energy boost, which helps power me through my day. I gain other benefits, including improved athletic performance, noticeably faster recovery from exercise, and an overall greater sense of well-being.

I highly recommend you try hydrogen supplements for yourself, and then consider them for your clients or patients. Our experience is that most people are pleasantly surprised by the results they get from hydrogen supplements, especially if they have a need for exceptional free-radical defense, or stand to benefit from the proven effects hydrogen uniquely provides (see list above).

Once you try hydrogen, you will want to continue taking it because the benefits are so significant. Let the power of hydrogen starting working for you on a daily basis!

hydrogen supplements Megahydrate

Dr. G. Patrick Flanagan’s Megahydrate (60 capsules).


Hydrogen Articles

Hydrogen for Optimal Health

Wonders of Molecular Hydrogen

Molecular Hydrogen (H2) at the Forefront of Health Research

The Science Behind MegaHydrate by Hank Liers, PhD

Hydrogen Products

Active H2 Ultra tablets

Vital Reaction tablets

Megahydrate capsules

Other Hydrogen Resources

Molecular Hydrogen Foundation

hydrogen supplements megahydrate active H2

Hydrogen supplements like Megahydrate and Active H2 are foundational in the HPDI supplement system.



Fred Liers PhD rejuvenate! plus superfoodAs if Rejuvenate! PLUS weren’t already the delicious, nutritious superfood you know and love. Now, Dr. Hank Liers, HPDI’s formulator, has enhanced the formula for a truly remarkable taste and nutrition experience, you must try for yourself to believe!

The name Rejuvenate! Plus remains, but think of it as “version 2.0” because the nutritional upgrades and taste improvements are striking.

By the way, the “new” Rejuvenate! Plus is available now, so you can look forward to a truly remarkable user experience. We are confident you will be more than pleasantly surprised.

You will not believe your tastebuds when you try the new Rejuvenate! Plus. My humble opinion? Dr. Hank (my father and HPDI’s formulator) has knocked it out of the park in his revised formula.

Reports from sample testers have been overwhelmingly positive! Individuals who loved Rejuvenate! Plus unanimously love the new formula even more. That says a lot about this reformulation.

Rejuvenate! Plus sweet green superfood

Rejuvenate! Plus 2.0 – healthy ingredients and taste at whole new level!

Other testers, who preferred our Rejuvenate! Berries & Herbs or Original Greens formulas either say they will now add Rejuvenate! Plus to their regimen, or will mix it in with their current favorite Rejuvenate! superfood.

Some individuals sampling the new formula who had never tried it before or previously did not often use Rejuvenate! Plus have decided to use it as their primary superfood—or take it daily.

We couldn’t be happier because these reports confirm the new Rejuvenate! Plus is a significant improvement over previous versions, and make our year-long effort to reformulate the product worth it. These early reports based on product samples show we have succeeded!

Some things haven’t changed: Rejuvenate! Plus remains the most popular and best-selling of all HPDI Rejuvenate superfoods. As always, it provide therapeutic levels (at least 340 mg) of dietary nucleic acids (RNA, DNA), greens (chlorella and spirulina) as in the lighter, sweeter greens (as compared to dark intense green-ness of Rejuvenate! Original greens) that both leads to slower, yet effective detoxification, and is powerfully energizing and cleansing. Rejuvenate! Plus also remains a foundational supplement that retains a complete built-in multivitamin sub-formula.

What then has changed? In short, we have changed a few ingredients, added or upgraded other ingredients, increased protein and nucleic acids (RNA) content, and created better tasting product overall. Here is a brief summary:

The “new” Rejuvenate! PLUS features (per serving):

1) 500 mg more spirulina (1,900 mg total), which actually increases the RNA content by about 15 mg

2) 2.3 grams more protein for a total of 13.9 grams

3) Almost no sugar (just 0.6 g total)

4) 2.3 g of powerful broccoli and alfalfa sprout extract powders

5) 200 mg of organic BiAloe® aloe vera powder with over 18% acemannan

6) 5-MTHF (500 mcg) — a better form of folate

6) An extremely smooth texture, and even more delicious taste—users rate as significantly better than prior versions—which says a lot because Rejuvenate! Plus already tasted great.

In short, these are the changes that make the new, improved Rejuvenate! Plus a significantly better product. Now I will delve a bit deeper into each of these changes, as well as specific changes to ingredients.

Rejuvenate! Plus superfood

The new Rejuvenate! PLUS offers major improvements for optimal health.


Rejuvenate! Plus is one three high-RNA superfoods Hank Liers of HPDI intentionally formulated to provide therapeutic levels of dietary nucleic acids. In this case, that means about 340 mg of nucleic acids per serving, and that has now been increased to at least 355 mg. The fact that Rejuvenate! Plus provides such high levels of dietary nucleic acids makes it—and the entire line of Rejuvenate! superfoods—unique!

A short history lesson: Benjamin S. Frank, MD, PhD discovered the benefits of dietary nucleic acids in the 1960s and 1970s. He performed various studies on humans and animals using various intakes of dietary RNA. He concluded that nucleic acids are essential nutrients like vitamins and minerals. Nucleic acids provide a host of unique health benefits that make getting RNA in your diet important.

After reading of Dr. Benjamin S. Frank’s works (including Dietary Nucleic Acid Nutrition and Therapy and The No-Aging Diet), my father Hank Liers and I decided to formulate a line of superfoods based on the idea of providing high levels of dietary nucleic acids for optimal health. That was 10 years ago.

Rejuvenate! Original Greens launched in 2007. Rejuvenate! Plus came two years later in 2009. Then Rejuvenate! Berries & Herbs in 2010. To this day, no other company has created dedicated high-RNA superfoods like HPDI’s Rejuvenate line.


What makes Rejuvenate! PLUS—and in fact the entire Rejuvenate! line of superfoods—unique? A single serving provides therapeutic levels (340+ mg) of dietary nucleic acids (RNA, DNA, nucleotides, and nucleosides). Here are a few of the benefits and effects of nucleic acids in the body:


Source: Nucleic Acid Nutrition and Therapy

Nucleic acids perform so many important functions in the body that getting sufficient amounts in the your diet is probably essential, just as Dr. Benjamin S. Frank asserts. That can be difficult since historically they were obtained from fish, organ meats—foods that people today may not consume in sufficiently high amounts for a variety of reasons. These reasons may include issues of taste, emphasis on vegetarian foods, concern about heavy metals or radiation, or lack of availability.

Rejuvenate! Plus in many ways helps take the guesswork from obtaining “essential” dietary nucleic acids by providing a vegetarian, gluten free, and non-GMO source of dietary RNA—along with protein, vitamins, minerals, cofactors, and other important nutrients—in a tasty, easy-to-take powder that stays fresh for months, travels well, and can be taken in a variety of ways from smoothies to plain water!


You (or your clients) have everything to gain by trying Rejuvenate! PLUS. As you can see, the updated superfood is carefully formulated to help create and maintain optimal health.

When used together with other HPDI foundational supplements and the HPDI supplement system, Rejuvenate! Plus is another “brick in your wall” of good health and can make a huge difference in terms of how you feel, perform, and function in life.

Try the new, improved Rejuvenate! PLUS “2.0” superfood. See for yourself not only how good it tastes, but how good it can help make you feel. That’s goodness all around!



Nine Things to Know About Rejuvenate Superfoods

Dietary Nucleic Acids – Dr. Benjamin S. Frank, Part 1

Dietary Nucleic Acids – Dr. Benjamin S. Frank, Part 2

Super-Tasty Morning Nutritional Drinks

Product Reviews

Rejuvenate! PLUS Superfood is High in DNA and RNA with sprouts, chlorella & more by Mike Adams

Product Review: Rejuvenate Berries & Herbs superfood is rich in RNA, plant proteins by Mike Adams

Superfood for Cellular Survival by Dr. Mark Sircus


Nucleic Acid Nutrition and Therapy by Benjamin S. Frank, MD, PhD

Dr. Frank’s No-Aging Diet: Eat and Grow Younger by Benjamin S. Frank, MD, PhD

Nucleic Acid and Anti Oxidant Therapy of Aging and Degeneration by Benjamin S. Frank, MD, PhD

Nucleic Acid Therapy in Aging and Degenerative Disease–A Metabolic Approach with DNA, RNA, and Related Metabolites by Benjamin S. Frank, MD, PhD

Rejuvenate! Superfoods

Rejuvenate! Superfoods

Rejuvenate! comparison page

Rejuvenate! PLUS

Rejuvenate Berries & Herbs

Rejuvenate! Original Greens




Dr. Hank Liers, PhD pro-c™ pro-c super antioxidant formulaFred Liers PhD pro-c antioxidant vitamin c nrf2 formulaLooking for an advanced antioxidant formula? Already using or recommending vitamin C? Curious about cellular Nrf2 activation? Look no further than PRO-C™.

PRO-C™ is among the most effective antioxidant formulas available. It is an HPDI foundational supplement that works most effectively when used with multivitamins, essential fats, and superfoods. However, it is also an excellent standalone formula that can rapidly provide the body with extremely high protection from free radicals.

We ourselves have taken PRO-C daily for many years with excellent results. Our personal experience together with detailed feedback from health professionals and end-users affirms the effectiveness of PRO-C as a super-antioxidant–vitamin C-Nrf2 activator formula.

PRO-C provides 500 mg of buffered vitamin C per capsule (buffered with calcium, magnesium, and zinc) along with grape extract (seed, skin, pulp) and green tea extract (95% polyphenols). In addition, we include a special combination of the “network antioxidants” l-glutathione (reduced), n-acetyl-l-cysteine (NAC), r-lipoic acid, and selenium. Vitamin B2 and Vitamin B6 in coenzyme forms support the enzymatic effectiveness of the “network antioxidants”. The formula works so well because this combination of ingredients leverages the antioxidant power of vitamin C, grape extract, green tea extract, and the other nutrients to act synergistically in order to maximize effectiveness.


What you may not know is the history of the development PRO-C and the scientific knowledge on which Dr. Hank Liers based his formulation of it.

Dr. Hank formulated his first product in 1989. It was a potent antioxidant formula he called PYC-C™ (sounds like “pixie”). PYC-C consisted of a combination of buffered Vitamin C (including magnesium, calcium, and zinc ascorbates) and pycnogenols from pine bark.

Much of the scientific research data Dr. Hank collected during the development of PYC-C regarding oligomeric proanthocyanidins (OPC) he later incorporated into an article (currently published on this blog) titled “Review of Scientific Research on Oligomeric Proanthocyanidins (OPC)” (rev. 2017)

By 1997 Dr. Hank had gathered a great deal of new scientific information regarding green tea catechins and the nutrients termed “network antioxidants” by Dr. Lester Packer, director of Packer Lab at University of California, Berkeley. Beyond this information, Dr. Hank studied additional research regarding how various nutrients worked together synergistically. At that point, he was ready to formulate the new, improved PRO-C™ super antioxidant formula.

PRO-C combines the ingredients of PYC-C (now known as OPC-C™) and uses grape pulp, skin, and seed extract with green tea extract (with high polyphenols >95% and EpiGalloCatechinGalate (EGCG) >45%), n-acetyl-l-cysteine (NAC), reduced glutathione (GSH), R-lipoic acid, selenium, and coenzyme Vitamins B2 and B6.

PRO-C super antioxidant formula 180 cap 90 cap

HPDI launched PRO-C™ in late 1997. It rapidly became one of our best-selling products. Our customers raved about how effective it was for them if they felt like they were “coming down with something” (like a cold, flu, virus, infection, etc.). Greater skin elasticity greatly helped pregnant women avoid stretch marks and episiotomies. Today, we highly recommend its use together with our other Foundational Supplements to ensure optimal health and anti-aging effects.


PRO-C™ super antioxidant formula is extremely synergistic, especially in so far as it increases the body’s ability to quench free radicals in its aqueous (i.e., water-based) compartments. Because antioxidants may become free radicals themselves after they have done their job, the body has developed an elaborate system for recovery of oxidized antioxidants.


Dr. Lester Packer was the primary researcher investigating the synergistic character of antioxidants. He made this statement in his interview with Dr. Richard Passwater after publication of Packer’s The Antioxidant Miracle (1999):

[The major theme of] The Antioxidant Miracle is that antioxidants work in a coordinated manner. They interact with one another, and this interaction, which we like to call the antioxidant network, is very important to the overall antioxidant defense that we possess. The key members of the antioxidant network are vitamin E and vitamin C, but there are other participants in this network. These are thiol antioxidants, antioxidants that contain sulfur groups in the body. Glutathione perhaps is the best known of these, but there are other sulfur-containing antioxidants that also are very important.”

Dr. Packer continues:

“This whole antioxidant network works like an orchestra depending on individuals who have, of course, different complements of antioxidants depending upon their nutritional regimens and the individuality of their own body metabolisms. The idea behind having a network of antioxidants is that if one antioxidant happens to be deficient the others can compensate and still keep the antioxidant defense system strong.”

The following diagram shows some of the relationships in the antioxidant network and how they support each other.

Lester Packer antioxidant network diagram Figure 1 – Dr. Packer’s Antioxidant Network

We see, for example, reduced glutathione (GSH) has the ability to reduce oxidized Vitamin C back to its unoxidized state. Vitamin C reduces oxidized Vitamin E back to its unoxidized state, and both reduces glutathione and spares it for other important functions, including detoxification and immune enhancement.

Many polyphenols (e.g., oligomeric proanthocyanidins (OPCs), anthocyanidins and catechins) found in red grape and green tea extracts spare Vitamin C and glutathione in the body, as well as operate as powerful antioxidants, anti-inflammatories, and connective tissue strengtheners.

grapes grape extract antioxidant

Grapes provide antioxidant nutrients such as polyphenols, OPCs, anthocyans, and resveratrol.

R-Lipoic Acid (see abstracts below) operates as an antioxidant both in its oxidized and reduced states, reduces the oxidized forms of both Vitamin E and Vitamin C, and and has been shown to enhance glutathione levels. Because several of these substances are able to protect Vitamin E contained in cell membranes, this combination also has a significant beneficial effect on the fat soluble antioxidant status of the body!

The nutrients in PRO-C have been carefully selected and balanced to provide optimal effects, especially as related to free radical protection, detoxification, immune system enhancement, connective tissue strengthening, and reduction of inflammation. PRO-C therefore provides outstanding nutritional support in a wide variety of conditions of poor health, as well as acts to support and maintain a state of health and well-being.

It the last several years the research results on Nrf2 activators have become well known and products developed that take advantage of these nutrients. For details see our blog article Natural Phytochemical Nrf2 Activators for Chemoprevention. Researchers have been studying specifically how enzyme-activating substances such as OPCs and anthocyans activate a transcription factor known as Nrf2 that causes the body to endogenously produce higher levels of a wide variety of protective enzymes including superoxide dismutase (SOD), catalase, and glutathione peroxidase.

Although we did not know about Nrf2 activators in 1997 when we formulated PRO-C, we have subsequently learned that four of the ingredients in the formula have powerful Nrf2 activity. These include grape seed extract, green tea extract, NAC, and r-lipoic acid. With this knowledge, we now understand that PRO-C provides both powerful external antioxidants (with extremely high ORAC5.0 values) that support redox cycles within the body, but also provides ingredients that allow the body to endogenously produce powerful protective enzymes for even greater free-radical protection and health.


PRO-C contains buffered vitamin C (in the form of powdered calcium, magnesium, and zinc ascorbates), high-potency grape extract (from grape pulp, skins, and seeds), green tea extract (with>95% polyphenols and >45% EGCG), reduced glutathione, N-Acetyl-L-Cysteine (NAC), R-lipoic acid, coenzyme forms of vitamin B2 (R5P) and vitamin B6 (P5P), and selenium.

Below we will discuss each ingredient and show some of the research that confirms its effectiveness.


Vitamin C typically is called l-ascorbic acid or ascorbate and is an essential nutrient for humans and other animal species. The term “vitamin C” refers to a number of vitamins that have vitamin C activity in animals, including ascorbic acid and its salts (e.g., magnesium ascorbate, calcium ascorbate, sodium ascorbate, etc.), and some oxidized forms such as dehydroascorbate and semidehydroascorbate.

Vitamin C is known to perform many critical functions within the body involving detoxification, tissue building, immune enhancement, pain control, and controlling or killing pathogenic organisms. It is also known to be helpful for wound and bone healing, healthy skin and eyes, fighting infections, stress control, toxic exposure, and repairing damaged tissue of all types. For much more information on the many benefits of Vitamin C see our blog article Vitamin C – An Amazing Nutrient.

Below are two abstracts that show some of the beneficial effects of Vitamin C when used with other network antioxidants:

Exhaustive physical exercise causes oxidation of glutathione status in blood: prevention by antioxidant administration.
Sastre J, Asensi M, Gasco E, Pallardo FV, Ferrero JA, Furukawa T, Vina J
In: Am J Physiol (1992 Nov) 263(5 Pt 2):R992-5

We have studied the effect of exhaustive concentric physical exercise on glutathione redox status and the possible relationship between blood glutathione oxidation and blood lactate and pyruvate levels. Levels of oxidized glutathione (GSSG) in blood increase after exhaustive concentric physical exercise in trained humans. GSSG levels were 72% higher immediately after exercise than at rest. They returned to normal values 1 h after exercise. Blood reduced glutathione (GSH) levels did not change significantly after the exercise. We have found a linear relationship between GSSG-to-GSH and lactate-to-pyruvate ratios in human blood before, during, and after exhaustive exercise. In rats, physical exercise also caused an increase in blood GSSG levels that were 200% higher after physical exercise than at rest. GSH levels did not change significantly. Thus, both in rats and humans, exhaustive physical exercise causes a change in glutathione redox status in blood. We have also found that antioxidant administration, i.e., oral vitamin C, N-acetyl-L- cysteine, or glutathione, is effective in preventing oxidation of the blood glutathione pool after physical exercise in rats.

The effect of glutathione and vitamins A, C, and E on acute skin flap survival.

Hayden RE, Paniello RC, Yeung CS, Bello SL, Dawson SM
In: Laryngoscope (1987 Oct) 97(10):1176-9

Vitamins A, C, and E act as antioxidants and as free radical scavengers in biological systems. Glutathione is involved in several reactions in vitamin metabolism and also plays an important role in cell membrane protection against lipid peroxidation by free radicals. We sought to use these natural defense mechanisms against oxygen free radicals formed during reperfusion of ischemic skin flaps. An acute axial random skin flap model was utilized in the rat. Vitamins or glutathione were administered by oral gastric tube or intravenously in the perioperative period, and survival of the flap was measured at 1 week. Glutathione, beta-carotene, ascorbic acid and alpha-D- tocopherol showed mean flap survival of 84% to 89%, each of which was significantly improved over saline controls (67% p less than .0005). The mechanisms and biochemistry of these vitamins, and their interactions with other vitamins and with glutathione, are discussed, along with clinical implications of free radical scavenging and skin flap survival.


Grape extract (seeds, skin, pulp) contain highly bioavailable bioflavonoid complexes that in research studies have been shown to have powerful antioxidant capability. The Oligomeric Proanthocyanidins (OPCs) in grape seed extract are able to strengthen collagen fibers in aging or damaged connective tissue and can act as a preventative against connective tissue degradation.

Some research indicates that anthocyans, which are found in extracts of grape skin and stems (but not in grape seed extract), can reduce oxidized glutathione while at the same time become reduced themselves. In addition, extracts of grape skin and stems (but not those of grape seed extract) contain a material called trans-resveratrol that has been shown to have chemopreventive effects.

Below we have provided some of the abstracts that are included in our broad list of relevant abstracts for PRO-C.

Protective effects of grape seed proanthocyanidins and selected antioxidants against TPA-induced hepatic and brain lipid peroxidation and DNA fragmentation, and peritoneal macrophage activation in mice.
Bagchi D, Garg A, Krohn RL, Bagchi M, Bagchi DJ, Balmoori J, Stohs SJ
In: Gen Pharmacol (1998 May) 30(5):771-6

1. The comparative protective abilities of a grape seed proanthocyanidin extract (GSPE) (25-100 mg/kg), vitamin C (100 mg/kg), vitamin E succinate (VES) (100 mg/kg) and beta-carotene (50 mg/kg) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced lipid peroxidation and DNA fragmentation in the hepatic and brain tissues, as well as production of reactive oxygen species by peritoneal macrophages, were assessed. 2. Treatment of mice with GSPE (100 mg/kg), vitamin C, VES and beta-carotene decreased TPA-induced production of reactive oxygen species, as evidenced by decreases in the chemiluminescence response in peritoneal macrophages by approximately 70%, 18%, 47% and 16%, respectively, and cytochrome c reduction by approximately 65%, 15%, 37% and 19%, respectively, compared with controls. 3. GSPE, vitamin C, VES and beta-carotene decreased TPA-induced DNA fragmentation by approximately 47%, 10%, 30% and 11%, respectively, in the hepatic tissues, and 50%, 14%, 31% and 11%, respectively, in the brain tissues, at the doses that were used. Similar results were observed with respect to lipid peroxidation in hepatic mitochondria and microsomes and in brain homogenates. 4. GSPE exhibited a dose-dependent inhibition of TPA- induced lipid peroxidation and DNA fragmentation in liver and brain, as well as a dose-dependent inhibition of TPA-induced reactive oxygen species production in peritoneal macrophages. 5. GSPE and other antioxidants provided significant protection against TPA-induced oxidative damage, with GSPE providing better protection than did other antioxidants at the doses that were employed.

Clinical and capillaroscopic evaluation of chronic uncomplicated venous insufficiency with procyanidins extracted from vitis vinifera
Costantini A, De Bernardi T, Gotti A
In: Minerva Cardioangiol (1999 Jan-Feb) 47(1-2):39-46

BACKGROUND: The pharmacological treatment of non-complicated chronic venous insufficiency is a current and well-debated topic. The introduction of new products with action on the venous system, improved knowledge on the physiopathology of venous insufficiency and the possibility provided by new analytical instruments, have given new impulse to the consolidation of the clinical value of phlebotonics in this indication. METHODS: In light of this, 24 patients with non-complicated chronic venous insufficiency were treated with oral administration of Oligomeric Proanthocyanidins (Pycnogenols-OPC) 100 mg/day. To evaluate the therapeutic efficacy of the treatment, an instrumental evaluation by optical probe capillaroscope was employed in addition to the traditional subjective clinical parameters: swelling, itching, heaviness and pain. The videocapillaroscope examination was performed at the lower third of the leg and the first toe. Edema in the capillaroscopic field, the number of observable capillaries and the capillary dilatation were the parameter chosen to evaluate the efficacy of treatment. All patients completed the study with no reports of adverse events during the period of observation. RESULTS: The results obtained show a positive clinical response (improved or absent symptoms) in over 80% of patients, with significant improvement of symptoms already evident after the first 10 days of treatment. The mechanism of action of the OPCs explains the rapid reduction of the swelling of the lower limbs and correlated with this are the other evaluable symptoms: heaviness and itching. Particularly striking results were observed for itching and pain which completely disappeared during the course of therapy in 80% and 53% of the patients respectively. Noteworthy is the good correlation between the clinical and instrumental data, with improvement in a total of 70% of patients. CONCLUSIONS: The results obtained in the course of this clinical experience, with evident improvement already during the first weeks of treatment, the absence of adverse events added to the benefit of a once-a-day administration, justify the use of OPC in the treatment of non-complicated chronic venous insufficiency.

Polymeric procyanidin fraction from defatted grape seeds protects HepG2 cells against oxidative stress by inducing phase II enzymes via Nrf2 activation.
Younghwa Kim, Youngmin Choi, Hyeonmi Ham, Heon-Sang Jeong, Junsoo Lee
Kim, Y., Choi, Y., Ham, H. et al. Food Sci Biotechnol (2013) 22: 485. https://doi.org/10.1007/s10068-013-0105-x

Nuclear factor erythroid 2-related factor 2 (Nrf2) is an important transcription factor that regulates antioxidant response element (ARE)-driven phase II detoxification enzymes. In this study, induction of phase II enzymes via Nrf2/ARE activation in the cytoprotective effect of crude polyphenol extract (CPE), oligomeric procyanidin fraction (OPF), and polymeric procyanidin fraction (PPF) from defatted grape seeds in HepG2 cells was evaluated. Among these treatments, the treatment with PPF significantly increased Nrf2 protein expression in the nuclear fraction. Treating the samples increased heme oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase 1 (NQO1) protein expression in a dose-dependent manner, and PPF significantly increased the levels of phase II enzymes. Cellular generation of reactive oxygen species (ROS) were effectively reduced by PPF. These results suggest that pretreatment with PPF shows a cytoprotective effect by inhibiting ROS production and inducing HO-1 and NQO1 expression via Nrf2 activation in HepG2 cells.


Green tea extract is obtained from the unfermented leaves of Camellia sinensis for which numerous biological activities have been reported including: antimutagenic, antibacterial, hypocholesterolemic, antioxidant, and protective against tumorigenesis. Below we have selected a few of the many abstracts we have on file showing the benefit of green tea extract.

Green tea antioxidant polyphenols catechins

Green tea leaves are high in antioxidant polyphenols and catechins.

Enhancement of antioxidant and phase II enzymes by oral feeding of green tea polyphenols in drinking water to SKH-1 hairless mice: possible role in cancer chemoprevention.
Khan SG, Katiyar SK, Agarwal R, Mukhtar H
In: Cancer Res (1992 Jul 15) 52(14):4050-2

Following the oral feeding of a polyphenolic fraction isolated from green tea (GTP) in drinking water, an increase in the activities of antioxidant and phase II enzymes in skin, small bowel, liver, and lung of female SKH-1 hairless mice was observed. GTP feeding (0.2%, w/v) to mice for 30 days significantly increased the activities of glutathione peroxidase, catalase, and quinone reductase in small bowel, liver, and lungs, and glutathione S-transferase in small bowel and liver. GTP feeding to mice also resulted in considerable enhancement of glutathione reductase activity in liver. In general, the increase in antioxidant and phase II enzyme activities was more pronounced in lung and small bowel as compared to liver and skin. The significance of these results can be implicated in relation to the cancer chemopreventive effects of GTP against the induction of tumors in various target organs.

In: Anticancer Drugs (1996 Jun) 7(4):461-8
Institutional address: Department of Pharmacology and Toxicology College of Pharmacy University of Arizona Tucson 85721 USA.

Green tea is an aqueous infusion of dried unfermented leaves of Camellia sinensis (family Theaceae) from which numerous biological activities have been reported including antimutagenic, antibacterial, hypocholesterolemic, antioxidant, antitumor and cancer preventive activities. From the aqueous-alcoholic extract of green tea leaves, six compounds (+)-gallocatechin (GC), (-)-epicatechin (EC), (-)- epigallocatechin (EGC), (-)-epicatechin gallate (ECG), (-)- epigallocatechin gallate (EGCG) and caffeine, were isolated and purified. Together with (+)-catechin, these compounds were tested against each of four human tumor cells lines (MCF-7 breast carcinoma, HT-29 colon carcinoma, A-427 lung carcinoma and UACC-375 melanoma). The three most potent green tea components against all four tumor cell lines were EGCG, GC and EGC. EGCG was the most potent of the seven green tea components against three out of the four cell lines (i.e. MCF-7 breast cancer, HT-29 colon cancer and UACC-375 melanoma). On the basis of these extensive in vitro studies, it would be of considerable interest to evaluate all three of these components in comparative preclinical in vivo animal tumor model systems before final decisions are made concerning which of these potential chemopreventive drugs should be taken into broad clinical trials.


Glutathione and NAC (a major precursor of glutathione) both provide important protection against toxins and free radicals, and can strengthen the immune system. Glutathione is considered to be one of the most important protective substances in the human body with almost 60% of liver detoxification accounted for by this key substance. In addition, glutathione is one of the most potent anti-viral substances known.

Some research has indicated that glutathione may not be able to enter easily into certain types of cells, but NAC is able to enter these cells and be converted into glutathione once inside the cell. Thus, the combination of glutathione and NAC appear to be more potent than either alone.

Below we provide some of the key abstracts we have on file regarding NAC and glutathione.

GSH rescue by N-acetylcysteine.
Ruffmann R Wendel A
In: Klin Wochenschr (1991 Nov 15) 69(18):857-62

Reduced glutathione (GSH) is the main intracellular low molecular weight thiol. GSH acts as a nucleophilic scavenger and as an enzyme-catalyzed antioxidant in the event of electrophilic/oxidative tissue injury. Therefore, GSH has a major role as a protector of biological structures and functions. GSH depletion has been recognized as a hazardous condition during paracetamol intoxication. Conversely, GSH rescue, meaning recovery of the protective potential of GSH by early administration of N-acetylcysteine (NAC), has been found to be life-saving. Lack of GSH and electrophilic/oxidative injury have been identified among the causes of the adult respiratory distress syndrome (ARDS), idiopathic pulmonary fibrosis (IPF), and the acquired immunodeficiency syndrome (AIDS). Experimental and early clinical data (in ARDS) point to the role of NAC in the treatment of these conditions. Recently, orally given NAC has been shown to enhance the levels of GSH in the liver, in plasma, and notably in the bronchoalveolar lavage fluid. Rescue of GSH through NAC needs to be appreciated as an independent treatment modality for an array of different disease, all of which have one feature in common: pathogenetically relevant loss of GSH.

Cysteine and glutathione concentrations in plasma and bronchoalveolar lavage fluid after treatment with N-acetylcysteine.
Bridgeman MM Marsden M MacNee W Flenley DC Ryle AP
In: Thorax (1991 Jan) 46(1):39-42

N-acetylcysteine (600 mg/day) was given to patients by mouth for five days before bronchoscopy and bronchoalveolar lavage to determine whether N-acetylcysteine could increase the concentrations of the antioxidant reduced glutathione in plasma and bronchoalveolar lavage fluid. Bronchoalveolar lavage was performed 1-3 hours (group 2, n = 9) and 16-20 hours (group 3, n = 10) after the last dose of N-acetylcysteine and the values were compared with those in a control group receiving no N-acetylcysteine (group 1, n = 8). N-Acetylcysteine was not detected in plasma or lavage fluid. Plasma concentrations of cysteine, the main metabolite of N-acetylcysteine and a precursor of reduced glutathione, were greater in the groups receiving treatment (groups 2 and 3) than in group 1. Cysteine concentrations in lavage fluid were similar in the three groups. Concentrations of reduced glutathione were greater in both plasma and lavage fluid in group 2 than in group 1. These data suggest that N-acetylcysteine given by mouth is rapidly deacetylated to cysteine, with resulting increases in the concentrations of cysteine in plasma and of reduced glutathione in plasma and the airways, which thus temporarily increase the antioxidant capacity of the lung.


R-Lipoic Acid is normally made at low levels in the human body, where it functions primarily as an important metabolic nutrient in the conversion of pyruvic acid into acetyl coenzyme A. As such, it plays a crucial role in the metabolism of both fats and carbohydrates into energy. In addition, r-lipoic acid functions as an extremely powerful antioxidant capable of trapping many different types of free radicals in the body.

Because it is both water and fat soluble, lipoic acid is able to operate in a broader range of body tissues than most other antioxidants. Its small size allows lipoic acid to enter areas of the body not easily accessible to many other substances; this allows lipoic acid, for example, to enter the cell nucleus and prevent free-radical damage to DNA.

Because it is such a powerful antioxidant and can easily function as such in both a reduced and oxidized state, lipoic acid is able to protect other important antioxidants such as glutathione, Vitamin E, and Vitamin C. R-lipoic acid is also able to chelate heavy metals such as lead, cadmium, mercury, free iron, and free copper out of the body.

Below we provide relevant scientific abstracts from our database regarding R-Lipoic acid.

Alpha-Lipoic acid as a biological antioxidant.
Packer L Witt EH Tritschler HJ
In: Free Radic Biol Med (1995 Aug) 19(2):227-50

alpha-Lipoic acid, which plays an essential role in mitochondrial dehydrogenase reactions, has recently gained considerable attention as an antioxidant. Lipoate, or its reduced form, dihydrolipoate, reacts with reactive oxygen species such as superoxide radicals, hydroxyl radicals, hypochlorous acid, peroxyl radicals, and singlet oxygen. It also protects membranes by interacting with vitamin C and glutathione, which may in turn recycle vitamin E. In addition to its antioxidant activities, dihydrolipoate may exert prooxidant actions through reduction of iron. alpha-Lipoic acid administration has been shown to be beneficial in a number of oxidative stress models such as ischemia-reperfusion injury, diabetes (both alpha-lipoic acid and dihydrolipoic acid exhibit hydrophobic binding to proteins such as albumin, which can prevent glycation reactions), cataract formation, HIV activation, neurodegeneration, and radiation injury. Furthermore, lipoate can function as a redox regulator of proteins such as myoglobin, prolactin, thioredoxin and NF-kappa B transcription factor. We review the properties of lipoate in terms of (1) reactions with reactive oxygen species; (2) interactions with other antioxidants; (3) beneficial effects in oxidative stress models or clinical conditions.

Regeneration of glutathione by α-lipoic acid via Nrf2/ARE signaling pathway alleviates cadmium-induced HepG2 cell toxicity.
Zhang J, Zhou X, Wu W, Wang J, Xie H, Wu Z.
In: Environ Toxicol Pharmacol. 2017 Apr;51:30-37. doi: 10.1016/j.etap.2017.02.022. Epub 2017 Feb 27.

Alpha-lipoic acid (α-LA) is an important antioxidant that is capable of regenerating other antioxidants, such as glutathione (GSH). However, the underlying molecular mechanism by which α-LA regenerates GSH remains poorly understood. The current study aimed to investigate whether α-LA regenerates GSH by activation of Nrf2 to alleviate cadmium-induced cytotoxicity in HepG2 cells. In the present study, we found that cadmium induced cell death by depletion of GSH through inactivation of Nrf2. Addition of α-LA to cadmium-treated cells reactivated Nrf2 and regenerated GSH through elevating the Nrf2-downstream genes γ-glutamate-cysteine ligase (γ-GCL) and GR, both of which are key enzymes for GSH synthesis. However, blocking Nrf2 with brusatol in the cells co-treated with α-LA and cadmium reduced the mRNA and the protein levels of γ-GCL and GR, thus suppressed GSH regeneration by α-LA. Our results indicated that α-LA activated Nrf2 signaling pathway, which upregulated the transcription of the enzymes for GSH synthesis and therefore GSH contents to alleviate cadmium-induced cytotoxicity in HepG2 cells.


Selenium has been shown by clinical research to be a key mineral in the body’s defenses against free radicals and has been shown to be a major factor in reducing the symptoms of HIV infections and in the prevention of tumors. Selenium is used in conjunction with glutathione to form the powerful enzyme glutathione peroxidase that is responsible for detoxification of peroxides formed during the process of aerobic metabolism in humans and other animals.

Serum selenium concentrations in rheumatoid arthritis.
In: Ann Rheum Dis (1991 Jun) 50(6):376-8

O’Dell JR, Lemley-Gillespie S, Palmer WR, Weaver AL, Moore GF, Klassen LW

Selenium is a trace element and an essential part of the enzyme glutathione peroxidase, which protects cells from oxidative damage. Selenium has been shown to have antiproliferative, anti-inflammatory, antiviral, and immune altering effects. Serum selenium concentrations in 101 patients with seropositive rheumatoid arthritis were found to be significantly lower than those in 29 normal, healthy controls (mean (SD) 148 (42) v 160 (25) micrograms/l) and also lower than those in eight patients with fibrositis (148 (42) v 166 (25) micrograms/l). It is speculated that serum selenium concentrations may modulate the effect of viral or other infections in subjects with the appropriate genetic background and in this way enhance the development or progression of rheumatoid arthritis.

Studies on selenium in top athletes.
Dragan I, Ploesteanu E, Cristea E, Mohora M, Dinu V, Troescu VS
In: Physiologie (1988 Oct-Dec) 25(4):187-90

The authors performed a controlled trial in 18 top athletes (9 weight lifters and 9 rowers, girls) in order to make evident some chronic and acute effects (antioxidant) of selenium. Nonprotein–SH (essential glutathione), lipid peroxides (MDA-malondialdehyde), glucose-6-phosphate dehydrogenases (G-6-PDH) and fructose-1,6- diphosphate aldolase in serum, have been recorded initially on basal conditions, after 3 weeks of treatment (100 micrograms/day selenium or placebo) and again after 3 weeks of treatment, also on basal conditions, when crossing over the groups (between a free interval of 10 days). In another trial we registered these parameters on basal conditions and after two hours of hard training accompanied by a per oral administration of 150 micrograms selenium (respectively placebo). The results show significant changes under selenium treatment of the peroxides, G-6-PDH and light changes, not significant of the nonprotein–SH, changes which could suggest an antioxidant effect of this element.


Vitamin B2 as coenzyme riboflavin-5-phosphate is a key vitamin that supports the regeneration of glutathione (via glutathione reductase). Vitamin B6 as coenzyme pyridoxal-5-phosphate is a key vitamin that supports the ability of glutathione to combine with toxic substances (via glutathione transferase) in the process of eliminating them from the body. They are especially effective in their coenzyme forms which allows them to be directly utilized by the body starting in the intestinal tract.


Magnesium, zinc, and calcium synergistically work with (and enhance the effects of) the other ingredients in PRO-C. Minerals are especially needed as active components of enzymes that drive metabolic activity. For example, magnesium is required in the functioning of more than 325 types of enzymes.


HIGHLY EFFECTIVE VITAMIN C FORMULA PLUS ANTIOXIDANTS. A complete vitamin C formula, a powerful antioxidant Formula, and Nrf2 activator combined in a single advanced supplement!

POWERFUL, SYNERGISTIC FREE-RADICAL QUENCHING FORMULA. PRO-C™ components work together to quench free radicals in your body. Vitamin C enables grape seed extract to function more effectively, and conversely grape seed extract potentiates vitamin C. Green tea extract boosts ORAC (Oxygen Radical Absorbance Capacity) value.

PROVIDES SIGNIFICANT AMOUNTS OF POWERFUL NRF2 ACTIVATORS (from Grape Extract, Green Tea Extract, NAC, and R-Lipoic Acid) that stimulate the production of the body’s own protective antioxidants including superoxide dismutase, catalase, glutathione peroxidase, and heme oxygenase.

SUPERIOR, BUFFERED (NON-ACIDIC) FORM OF VITAMIN C. Mineral Ascorbates never acidify your body, keeping you pH balanced. Staying alkaline is an important element in maintaining a healthy body.

RAPID ASSIMILATION. Capsule form ensures rapid uptake and assimilation in the body. You may also empty capsule contents into water, food, or directly Into mouth, if desired. Good, mildly tart taste!


One (1) vegetarian capsule of PRO-C provides the following percentages of the Daily Value:

Vitamin C (from mineral ascorbates) 500 mg 833%
BioVin® Grape Extract 30 mg *
Green Tea Extract 30 mg *
Calcium (from calcium ascorbate) 23 mg 2.3%
Magnesium (from magnesium ascorbate) 23 mg 5.7%
L-Glutathione (reduced) 20 mg *
N-Acetyl-L-Cysteine (NAC) 15 mg *
R-Lipoic Acid 5 mg *
Zinc (from zinc ascorbate) 2 mg 13%
Vitamin B2 (from riboflavin-5′-phosphate) 1 mg 118%
Vitamin B6 (from pyridoxal-5′-phosphate) 1 mg 50%
Selenium (from l-selenomethionine) 10 mcg *

* No established Daily Value

DIRECTIONS: As a dietary supplement take 1–3 capsules or more daily in divided doses (i.e., spread out over the day), or as recommended by a health care professional. It initially may be useful to take up to 6 capsules per day in divided doses for one week. The contents of the capsule may be emptied into juice or food, as needed.

INGREDIENTS: PRO-C™ SUPER ANTIOXIDANT FORMULA contains only the highest-quality USP grade magnesium ascorbate, USP grade calcium ascorbate, BioVin® grape extract (greater than 75% polyphenols, 93% OPC, greater than 3.5% anthocyanidins from grape pulp, skins, and seeds, and a small amount of trans resveratrol), green tea extract (95% min. polyphenols and 45% min. EGCG), l-glutathione (reduced), USP grade n-acetyl-l-cysteine, USP grade zinc ascorbate, r-(+)-lipoic acid, riboflavin-5′-phosphate, pyridoxal-5′-phosphate, l-selenomethionine, the smallest amounts of microcrystalline cellulose and silica in a vegetarian capsule.

PRO-C™ does not contain wheat, rye, oats, corn antigen, barley, gluten, soy, egg, dairy, yeast, sugar, sulfates, phosphates (other than coenzyme forms), fats, chlorides, GMOs, wax, preservatives, colorings, or artificial flavorings.

Click here to order PRO-C™.



The Antioxidant Miracle. Lester Packer, PhD, and Carol Coleman. New York: John Wiley and Sons, 1999.

How to Live Longer and Feel Better. Dr. Linus Pauling. Corvallis, OR: Oregon State University Press, 2006.


Review of Scientific Research on Oligomeric Proanthocyanidins (OPC)” (rev. 2017) by Hank Liers, PhD

“Vitamin C – An Amazing Nutrient” by Hank Liers, PhD

PRO-C™ and Ultimate Protector™ – Comparison by Hank Liers, PhD

“Antioxidant Cocktail Update: Part 1: The Take Home Message is to Use Antioxidant Supplements”
(An interview of Dr. Lester Packer by Richard A. Passwater, PhD, Whole Foods Magazine 1999)


PRO-C™ / Vitamin C Abstracts

Catechin Abstracts

N-Acetyl-L-Cysteine (NAC) Abstracts

Lipoic Acid Abstracts


(Therapeutic Nutrition Based Upon Biochemical Individuality)


PRO-C™Super Antioxidant Formula

Ultimate Protector™Nrf2 Activator Formula


HPDI Vitamin C Products



Fred Liers PhD intestinal rejuvenation formula IRFAmong the most essential aspects of detoxification are the sequestering of toxins and waste from the gastrointestinal tract and its proper elimination. HPDI’s Intestinal Rejuvenation Formula meets the need for an effective intestinal sweep to cleanse the entire GI tract for improved gut health and function.

Intestinal Rejuvenation Formula (or “IRF”) is our unique product that 1) latches onto toxins, old waste, and deposits in the digestive tract, 2) binds, pulls, and absorbs these materials, 3) sweeps them from the entire intestinal tract, while 4) naturally and gently facilitating elimination.

That’s just the start! You have to experience this formula yourself to appreciate its benefits most.

Intestinal Rejuvenation Formula works by coating and adhering to the lining of the intestines. This unique action allows it to absorb fecal plaques and other debris, soften unhealthy deposits, and sweep them away for elimination in the stool. The IRF formula thereby neutralizes and removes hundreds of toxins, including heavy metals like mercury and lead. It draws out many times its weight in material encrusted or trapped in the intestinal lining. It also provides nutrients not only known to nourish the GI tract, but also that promote normal elimination functions.

The easiest way to start using Intestinal Rejuvenation Formula is to put one teaspoon in eight ounces of water. Stir or shake, then drink immediately. You can then add some additional water to the container, stir and drink. You can start at 1/2 teaspoon (or less) if one teaspoon seems like too much. You can gradually work you way up to one tablespoon in 16 ounces of water or juice.

Note: It is important to consume the formula immediately after mixing with water to avoid the formula thickening which makes it difficult to drink. It is also important to consume sufficient water so it can swell and more easily pass through the digestive tract.

Intestinal Rejuvenation Formula

Intestinal Rejuvenation Formula is formulated to cleanse, detoxify, purify, and heal. You and your clients will love IRF!


Intestinal Rejuvenation Formula relieves symptoms of constipation, including gas, bloating, cramps, indigestion, and nausea caused by excessive waste in the bowel. It also soothes the intestinal lining and colon wall, and promotes a well-functioning colon. This means it can contribute to greater health not only through removal of toxins and intestinal blockages, but also by allowing greater absorption of nutrients from food (i.e., after the intestines are cleansed). It also can contribute to achieving normal weight by promoting elimination of old fecal matter (e.g., post-putrefactive deposits), and by helping to establish and maintain normal bowel function.

Intestinal Rejuvenation Formula acts as a wonderful soothing agent for the mucous membrane linings of the entire intestinal tract, especially the colon. This makes it an important formula for individuals experiencing irritable bowel syndrome, spastic colon, colitis, Crohn’s disease, diverticulitis, or any digestive tract condition causing hot, burning, irritated, or bleeding bowels and/or diarrhea. This unique healing formula is also an excellent remedy for food poisoning, because it can absorb harmful bacteria and toxins.

Intestinal Rejuvenation Formula also can be applied externally as a poultice to insect bites and stings (e.g., bee, ant, scorpion), snake bites, etc. The drawing power of the formula acts to pull toxins directly from wounds. Poultices are highly effective for pulling venoms, and work especially well when combined with echinacea tincture (used orally and topically) and homeopathic remedies (e.g., apis mellifera, ledum palustre, arnica montana). This application makes the formula truly unique. See directions (below) for external applications.


The ingredients in Intestinal Rejuvenation Formula make it one of the most effective products for complete cleansing of the gastrointestinal tract available today. IRF includes not only includes ingredients for binding and removing toxins and waste, but also for healing the entire GI tract.

INGREDIENTS: One teaspoon (3.23 gram) provides the following nutrients:

Psyllium Husk Powder (organic) – 1,000 mg
Apple Pectin – 400 mg
Bentonite Clay (wildcrafted) – 400 mg
Cold-Milled Flax Seed Powder (organic) – 400 mg
Marshmallow Root Powder (organic) – 200 mg
Slippery Elm Bark Powder (organic) – 200 mg
Activated Willow Charcoal (wildcrafted) – 100 mg
Spirulina Pacifica – 100 mg
Inulin / Fructooligosaccharides (from Chicory Root) – 100 mg
Ginger Root Powder (organic) – 100 mg
Natural Lemon Flavor – 100 mg
Vitamin C (from magnesium ascorbate) – 60 mg
Malic Acid – 50 mg
Grape Extract (pulp, skin, & seeds) – 40 mg
Licorice Root Powder (organic) – 40 mg
Stevia Leaf Extract (97% Rebaudioside A) – 13 mg
Magnesium (from magnesium ascorbate) – 4.5 mg
Chlorella Growth Factor (CGF) – 4 mg

Intestinal Rejuvenation Formula

The nutrients contained in Intestinal Rejuvenation Formula are all known to provide unique therapeutic benefits and healing properties. Consequently, the formula is carefully designed so the ingredients work together synergistically to leverage and maximize the capacities of each one. This results in a formula that is superior in action and that can far more effectively support health and healing in the intestinal tract than single ingredients or shotgun approaches (that often result in reduced effectiveness through the use of fillers, imbalanced formulation, or less than the highest quality ingredients).

Intestinal Rejuvenation Formula is a superior formulation representing a higher level of design expertise. This unique formula can offer significant benefits and results for individuals who wish to improve, regenerate, rejuvenate, heal, and sustain excellent gastrointestinal health.


Psyllium seed husks are soluble in water, expanding and becoming mucilaginous when moistened. Psyllium seed husks are indigestible in human beings and are used as a source of dietary fiber. They are used to relieve constipation, irritable bowel syndrome, diverticular disease, and diarrhea. They are also used as a regular dietary supplement to improve and maintain regular gastrointestinal (GI) transit. The inert bulk of the husks helps to provide a constant volume of solid material regardless of other aspects of the diet or any disease condition of the gut. Recent research also shows psyllium seed husks to be promising in lowering cholesterol and controlling diabetes.


As a dietary fiber, apple pectin is helpful in maintaining good digestive health. Pectin forms a colloidal solution in water and gels when cool. An indigestible, soluble fiber, apple pectin is a general intestinal regulator used in many healing preparations, especially as an anti-diarrheal agent. Researchers at the University of California, Davis (UCD), found that apple pectin acts as an antioxidant against the damaging portion of cholesterol in the blood stream.

It has been established that a diet rich in apple pectin may help protect against certain diseases. Research in Japan shows that apple pectin can act as a chemopreventive agent. Apple pectin helps maintain intestinal balance by cleansing the intestinal tract with its soluble and insoluble fibers. Apple pectin tends to increase acidity in the large intestines and is advocated for those suffering from ulcer or colitis and for regulating blood pressure. Pectin is also effective in causing regressions in and preventing gallstones. There is also evidence that the regular use of apple pectin may lessen the severity of diabetes.

Apple pectin is known to draw radiation from the body. Russian doctors have found apple pectin to be among the most effective means for protecting against radiation when consuming contaminated foods is unavoidable. Pectin, along with vitamins and minerals, eliminates radionuclides ingested from foods. This is especially relevant given recent meltdowns and radiation releases from the nuclear complex in Fukushima, Japan. Radiation from this catastrophe has contaminated (and continues contaminating) food crops across the entire northern hemisphere (and beyond), especially crops grown in North America.


Bentonite clay has a large and varied mineral content, including over 50 minerals. Seen under a high powered microscope, bentonite is composed of extremely minute rectangular particles. The wide surfaces are negatively charged and the edges are positively charged. Therefore, its adsorptive power is many thousand times more negative than positive. It has a negative electrical attraction for positively charged particles. In the human body, various toxic poisons are positively charged. The very minuteness of the particles give it a large surface area (about 1,000 square meters per gram) relative to its volume, thereby enabling it to pick up many times its own weight (as high as 40 times) in positively charged particles.

When bentonite clay absorbs water and swells, it stretches open like a highly porous sponge. Toxins (including pathogenic viruses, herbicides, and pesticides) are drawn into these spaces and bound through electrical attraction. The bentonite is eventually eliminated from the body with the toxins bound to its multiple surfaces. Diarrhea can be remedied through the use of bentonite clay because of its capacity to bind stools. Bentonite clay can act immediately to bind irritants in the gastrointestinal tract.

COLD-MILLED FLAX SEEDS (organically grown)

Flax Seeds (Linum usitatissimum L.) contain essential fatty acids (an excellent vegetarian source of omega-3 alpha-linolenic acid), protein, soluble fiber, insoluble fiber, antioxidant lignans, minerals, and vitamins. Lignans are a phytoestrogens considered to be antioxidants. Flax seeds contain among the highest levels of lignans among all plant foods.

The unique properties of Heartland’s RCM™ Flax (Real Cold Milled) organic flaxseed provides enormous advantages over whole flax seeds, ground flax powders, and other milled flax seed products. In particular, cold-milled flax seeds offer readily assimilable omega-3 and omega-6 fatty acids because of special no-heat processing that protects these delicate oils and makes them available for uptake into the body.

RCM™ Flax cold-milled flaxseed is processed using a unique, proprietary method that preserves the delicate oils and allows for optimal uptake of the oils and other nutrients (e.g., soluble fiber) into the body. That is, flax seeds must be milled into a flour or meal to obtain maximum benefits from its oils and dietary fiber. However, typical methods of grinding or pounding flaxseeds separate the oil from the seeds (and heat the oils), thereby causing the oils to oxidize and become rancid. In contrast, cold milling is a unique process that does not involve heating delicate oils and keeps them protected within finely milled pieces of seed. Thus, cold-milled flax seed is not significantly oxidized and remains fresh for an extended period of time. Conversely, when flax seeds are harshly ground or crushed, the oils do not remain fresh because there is no inherent protection from oxidation. Cold milling allows the seed to retain and protect its oils because the process does not release the oils from the seeds.

RCM™ Flax offers a non-refrigerated shelf life of greater than 24 months. A study conducted at North Dakota State University confirms that peroxide levels of RCM™ Flax remain exceptionally low for an extended periods. While this flaxseed retains its freshness for an extended period, when refrigerated (or consumed within several months), it provides amazingly high levels of freshness.

The organic cold-milled flax seeds in Intestinal Rejuvenation Formula contain oil amounts varying between 38–45%, with the oil offering a high percentage (47–53%) of the omega-3 alpha-linolenic acid (ALA) and 15–18% omega-6 linoleic acid. Alpha-linolenic acid (ALA) is an essential fatty acid acting in the human body as a substratum for the transformation into EPA and DHA through the action of desaturation and elongation enzymes. The seeds also contain some omega-6 essential fatty acids and exhibit a favorable omega-3 to omega-6 ratio of 3:1. Modern diets contain too few omega-3 fatty acids.

Cold-milled flax seeds possess 15–30% protein (typically 20%) and the amino acids of flax protein have no limiting factors in the adult diet (i.e., they are a complete protein source having all of the essential amino acids in an appropriate balance). Flax seeds are also a source of magnesium, calcium, potassium, phosphorus, zinc, and copper. They also have demonstrated antioxidant activity. The most important antioxidants they provide are phytoestrogens known as lignans. Because cold milling flax seeds significantly reduces oxidation, this exceptional flax seed has great potential within the food industry when compared to other alpha-linolenic acid sources, such as typical ground flaxseed powders, which can exhibit rapid decomposition due to a lack of antioxidant protection from oxidation.

The human body easily digests flax seeds. The seed’s outer layer is rich in mucilloid soluble fiber (flax seed possesses 10-15% soluble fiber) and absorbs at least 4 times its weight in water. When mixed with water or stomach juices, the seeds form a gel that creates a physical barrier between the carbohydrates and the digestive enzymes that break them down. The carbohydrates thereby are digested and converted into glucose (blood sugar) at a slow, uniform rate. There is no insulin surge or spike needed to lower the blood sugar level. The water-retaining capacity of the gel also maintains bodily hydration (i.e., helping level out water intake) and electrolyte balance.


Considered the king of mucilaginous (gel-like) slippery plants, marshmallow offers a unique composition of about 37% starch, 11% mucilage, and 11% pectin (including flavonoids and phenolic acids). When it reaches the colon, marshmallow gel will soften hardened fecal plaques and help to remove them from colon walls. It also acts as a soothing coating and internal moisturizer for the entire digestive tract. Like chia seeds, it neutralizes irritants often found in the intestinal system, and thereby greatly relieves irritation to the mucous membrane lining of the digestive tract.


The health benefits of slippery elm are rooted mainly in its soothing properties. This mucilaginous herb is effective when used internally for coating and soothing mucous membranes while also absorbing toxins that can cause intestinal imbalances. Slippery elm is an effective remedy for duodenal ulcers, gastritis, diarrhea, colitis, irritable bowel syndrome, hemorrhoids, and heartburn. The benefits of slippery elm also extend to being a simple food. As part of a normal diet, the inner bark of slippery elm can be ground and eaten as porridge. This porridge is extremely nutritious and high in antioxidants having a consistency and taste similar to oatmeal. It is also a wholesome and sustaining food for infants and invalids.


Activated charcoal is charcoal that has been treated with oxygen to open up millions of tiny pores between the carbon atoms. The use of special manufacturing techniques results in highly porous charcoals that have surface areas of 300–2,000 square meters per gram. Charcoal works to trap toxins through “adsorption.” When a material adsorbs something, it attaches to it via chemical attraction. The huge surface area of activated charcoal gives it countless bonding sites for adsorbing toxins.

When certain chemicals pass next to the carbon surface, they attach to the surface and become trapped. Activated charcoal is excellent for trapping carbon-based substances (i.e., organic chemicals), as well as other chemicals such as chlorine. It is known to trap over 20,000 organic chemicals. Many chemicals are not attracted to carbon (sodium, nitrates, etc.) so they pass through charcoal. Because charcoal is not “digested” like food, it may remain inside the GI tract for a time, thereby continuing to actively remove toxins via the bowel.


Research has shown that spirulina promotes digestion and bowel function. It suppresses bad bacteria (including E. coli and Candida albicans yeast) and stimulates beneficial flora, including lactobacillus and bifidobacteria. Healthy intestinal flora (i.e., “good” bacteria) are a foundation for good health. They increase absorption of nutrients from foods and help protect against infection. In Japan, researchers found that spirulina significantly reduces kidney toxicity caused by mercury and three pharmaceutical drugs. This suggests benefits for humans suffering heavy metal poisoning. Additional research found that rats consuming spirulina or chlorella algae eliminated seven times the amount of dioxin (a highly toxic chemical) when compared to a control diet.


Like other types of dietary fiber, inulin also has the benefits of enhancing digestive and intestinal functions, reducing cholesterol, and preventing disease. In addition, inulin has one other major health benefit: it is a prebiotic that feeds the healthy microflora that boost your immunity. Not only does inulin actively promote the growth of existing strains of probiotics in your colon, but it also encourages implantation, survival, and growth of freshly consumed probiotics. By boosting the survival and growth of beneficial probiotics in the inner ecosystem, inulin improves digestion, immunity, and disease-fighting capacity of the body.


Ginger rhizome (Zingiber officinale) is an incredibly active and effective gastrointestinal aid with the following properties: 1) Contains a digestive enzyme whose effectiveness even exceeds that of papain; 2) Stimulates the flow of saliva and increases the concentration of the digestive enzyme amylase in the saliva; and 3) Activates peristalsis and increases intestinal muscle tone. Ginger is also known to be helpful in cases of nausea.


BIOVIN® Grape Pulp, Skin & Seed Extract (>85% OPCs): The BioVin® grape extract in REJUVENATE!™ BERRIES & HERBS is extracted by ethanol and water and contains the highest percentage of OPCs (oligomeric proanthocyanidins) of any product on the market. OPCs, a class of bioflavonoids, are one of nature’s most powerful free-radical scavengers. As bioflavonoids, OPCs help to increase the effectiveness of Vitamin C. OPCs cross the blood-brain barrier to provide antioxidant protection to central nervous system tissue. Studies have clearly demonstrated that OPCs improve and normalize capillary activity, strengthening capillary walls. OPCs bind to collagen fibers, realigning them in the process to a more youthful, undamaged structure. Elasticity, flexibility, and strength are thereby restored to connective tissue.


Licorice root soothes the GI tract and aids intestinal health. Licorice root is used effectively in those individuals suffering from peptic ulcer and chronic gastritis. It is used to soothe coughs and reduce inflammation, soothe and heal ulcers and stomach inflammation, control blood sugar, and to balance hormones. Licorice is a potent antiviral agent and can be used to treat flu, herpes, and even hepatitis. Licorice is also a strong anti-inflammatory agent.

MAGNESIUM (from magnesium ascorbate)

Magnesium is important for the metabolism of many biologically active nutrients and substances, including calcium, potassium, phosphorus, zinc, copper, sodium, lead, cadmium, hydrochloric acid (HCl), acetylcholine, and nitric oxide (NO). It is required for more than 325 magnesium-dependent enzymes, cellular homeostasis, and activation of the B vitamin thiamin. It mediates the functions of the nervous and endocrine systems, supports proper muscle and nerve function, stabilizes heart rhythm, helps to regulate blood sugar levels, and supports normal blood pressure. Magnesium is important in energy metabolism and the synthesis of proteins.


Chlorella Growth Factor (CGF) stimulates lactobaccilus (the “good” bacteria) to grow at four times the usual rate. Experiments with microorganisms, young animals, and children have demonstrated that CGF promotes faster than normal growth. In adults and mature organisms, CGF appears to enhance the functions of nucleic acids at the cellular level that relate to the production of proteins, enzymes, and energy. It also stimulates tissue repair and offers protection against toxic substances at the cellular level.

VITAMIN C (from magnesium ascorbate)

Vitamin C is known to perform many critical functions within the body involving detoxification, tissue building, immune enhancement, pain control, and controlling or killing pathogenic organisms. It is known to be helpful for wound and bone healing, healthy skin and eyes, infections, stress control, toxic exposure, and repairing damaged tissue of all types.


Additional Ingredients: Natural Lemon Flavor, Malic Acid, and Stevia Leaf Extract. These natural flavors and taste enhancers give the formula a pleasing taste and make it easier for individuals to consume.

DIRECTIONS: For long-term, gentle rejuvenation of the intestinal tract take about one (1) teaspoon (about 1/3 scoop) several times daily away from food (by at least 20 minutes), or as directed by a health care professional. Place the powder in about 8 ounces of water, thoroughly mix (shake or stir), and drink immediately (before the formula becomes a gel too thick to easily consume).

For a more rapid healing cleanse take one tablespoon (one scoop) multiple times daily away from food (by at least 45 minutes). Add the powder to about 16 ounces of water, mix vigorously, and drink.

We recommend using a suitable container (such as a mason jar) for rapid mixing. Stir rapidly or shake vigorously (with lid attached).

Individuals suffering from conditions of severe diarrhea and loose stools can take one tablespoon (or more) multiple times daily (five times or more) and refrain from food. Put the powder in about 16 ounces of water per tablespoon, thoroughly mix, and drink immediately. In between servings it may be helpful to take a multiple strain lactic acid bacteria formula (i.e., a soil based probiotic such as Prescript-Assist). Place the formula directly in your mouth, chew, and then swallow.

While results can be obtained rapidly using this formula, it is most effective when used for longer-term gentle cleansing. Starting with lower doses and gradually increasing dosage often produces best results. This allows the body time to adjust to increased dosages (size and frequency) and facilitates greater therapeutic action from usage.

Note: Taking large amounts without sufficient liquid (e.g., water) or when first taking the formula can slow bowel transit time. Therefore, most individuals will benefit from starting at lower dosage levels and ensuring adequate fluid intake when consuming the formula.

External Applications: Make paste by mixing powder with water. Apply directly to skin or affected areas (e.g., stings, bites, wounds). Wrap with gauze, plastic, etc., and keep poultice moist. Use for up to six hours. Apply fresh poultice as needed.

Intestinal Rejuvenation Formula does not contain wheat, gluten, rye, oats, corn, barley, soy, yeast, egg, sugar, dairy products, GMOs, wax, animal fats, preservatives, colorings, or artificial flavorings.



Gut Health – Intestinal Rejuvenation Formula

Dr. Mark Sircus’s blog article at www.DrSircus.com:
“Apples – Pectin – Intestinal Formula”

Kidney Cleansing with Juices & Herbs

Liver Cleanse with Juices & Herbs

Rejuvenation Program: Part 2 (Detoxification)

Death by One (Or Two) Thousand Cuts

Microbiome, Diet, and Prescript-Assist Probiotic and Prebiotic

Chemical / Environmental Toxicity Syndrome Protocol

Super Remedies for Stings and Bites

Understanding Glyphosate Toxicity: Interview with Genetic Engineer Thierry Vrain (Mother Earth News)


The Colon Health Handbook by Robert Gray

Brain Maker: The Power of Gut Microbes to Heal and Protect Your Brain for Life by Dr. David Perlmutter

Grain Brain: The Surprising Truth About Wheat, Carbs, and Sugar – Your Brain’s Silent Killers by Dr. David Perlmutter

Wheat Belly: Lose the Wheat, Lose the Weight, and Find Your Path Back to Health by Dr. William Davis



PRESCRIPT-ASSIST™ Probiotic Formula
(soil-based organisms)

RESTORE For Gut Health

(proteolytic enzymes)

(full spectrum plant enzymes)

Nascent Iodine

Magnesium Oil, Flakes, Gel