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INTRODUCING A NEW HPDI WEBSITE FOR HEMP EXTRACTS AND CBD PRODUCTS: INTEGRATEDHEALTHHEMP.COM

Dr. Hank Liers, PhD new hemp CBD website integratedheathhempI am excited to announce Health Products Distributors is introducing a new website for high-quality hemp extracts and CBD formulas, including liposomal formulas from Quicksilver Scientific. Our new site is: IntegratedHealthHemp.com.

We at Health Products Distributors (HPDI) offer many types of nutritional supplements on our regular website (www.IntegratedHealth.com) since 1997. We devote our new hemp and CBD website to providing only top-of-the-line hemp extracts and CBD formulas.

The new website will carry a full range of highest-quality hemp extracts and CBD products. We currently offer two liposomal hemp extracts and three CBD Synergies products (all from Quicksilver Scientific, Inc.), and our brand new Soothe & Ease DMSO and hemp topical spray formula (from QTaneous). Here is a look at the current products on IntegratedHealthHemp.com.

BROAD SPECTRUM & FULL SPECTRUM HEMP EXTRACTS

QUICKSILVER NANOEMULSIFIED BROAD SPECTRUM HEMP EXTRACT 400 (0% THC):

Quicksilver Nanoemulsified Broad Spectrum Hemp Extract 400

• Superior nanoemulsified liposomal delivery. Quicksilver Delivery Systems™ phospholipid encapsulation system brings the power of intravenous delivery into convenient oral ingestion.

• 0% THC – Ideal for anyone subject to drug testing. The psychoactive part of the industrial hemp plant is totally removed (no THC) in this formula

• This delivery system improves upon liposomal technology with smaller, more stable, single-layer spheres made from the highest-grade ingredients available. In addition to exceptional absorption rates, nanospheres like the ones produced by Quicksilver Scientific have demonstrated the ability to cross the blood-brain barrier, deposit their cargo intracellularly, and enhance lymphatic circulation of nutrients.

• The phospholipids that compose the liposome shell feed the cell membranes helping ensure the proper function for the absorption of the nutrient and excretion of the cellular waste products and toxins

nanoemulsified broad spectrum hemp extract 400 Quicksilver Scientific

QUICKSILVER NANOEMULSIFIED FULL SPECTRUM HEMP EXTRACT 400 (<.3% THC):

Quicksilver Nanoemulsified Full Spectrum Hemp Extract 400

• Superior nanoemulsified liposomal delivery system allows for precise dosing and immediate effects. Quicksilver Delivery Systems™ phospholipid encapsulation system brings the power of intravenous delivery into convenient oral ingestion.

• Low THC (less than 0.3%). The small amount of THC (<0.3%) in this full spectrum product can actually postively enhance the functioning of the body’s endocannabinoid system. In this regard, the full spectrum product is preferable to the broad spectrum product, if you are not subject to drug testing for THC.

• Cannabidiol and associated components of the hemp extract interact with our body’s naturally occurring endocannabinoid system to aid with pain relief and enhanced feelings of well being.

• Due to known interaction of cannabidiol with endocannabinoid system receptors, much new research centers on cannabidiol’s receptor-mediated neuroprotective, antiemetic, and analgesic properties, and of its effect on mood and other aspects of mental health

• The delivery system improves upon liposomal technology with smaller, more stable, single-layer spheres made from the highest-grade ingredients available. In addition to exceptional absorption rates, nanospheres like the ones produced by Quicksilver Scientific have demonstrated the ability to cross the blood-brain barrier, deposit their cargo intracellularly, and enhance lymphatic circulation of nutrients.

• The phospholipids that compose the liposome shell feed the cell membranes helping ensure the proper function for the absorption of the nutrient and excretion of the cellular waste products and toxins

nanoemulsified full spectrum hemp extract 400 Quicksilver Scientific

CBD SYNERGIES PRODUCTS

CBD SYNERGIES-AX CALMING FORMULA

CBD Synergies-AX Calming Formula

cbd synergies calming formulas ax quicksilver scientific

CBD Synergies-SP Sleep Formula

CBD Synergies-SP Sleep Formula

• Soothing blend of nutraceuticals and botanicals supporting deep, restorative sleep

• PharmaGABA® and Melatonin help align the body with natural biological rhythms and support healthy sleep cycle

• Full spectrum hemp extract helps activate sleep-promoting brain pathways

• 5-HTP supports feelings of relaxation and wellbeing

• Skullcap and passion flower gently ease body into a restful slumber

CBD Synergies-PN Relief Formula

CBD Synergies-PN Relief Formula

• Full-spectrum hemp extract, a combination of CBD and a Farm Bill-compliant level of THC, regulates signaling pathways throughout the body to ease discomfort from head to toe

• Softgel capsule form self emulsifies in the body allowing for rapid effects via superior liposomal delivery

• Soothing effects are bolstered by β-caryophyllene, a unique hemp-based terpene

• Curcumin complex and boswellia extract work synergistically to alleviate gut and joint irritations, while piperine, an extract of black pepper, boosts the bioavailability of each element of our formula.

cbd synergies pain relief formula self emulsifying pn supplement

SOOTHE & EASE Formula (Topical DMSO and Hemp Extract)

QTaneous Soothe & Ease

• Unique spray topical with hemp extract and DMSO plus herbal ingredients

• Spray and/or rub directly on skin to support muscles, joints, and skin

• Acts rapidly and effectively to soothe and ease

• Ideal for home use, sports, or travel on-the-go

soothe and ease topical dmso hemp extract formula qtaneous

 

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TRANSCRIPT (partial)

Hello, this is Dr. Hank Liers from Health Products Distributors, Inc. (HPDI). Today we introduce a new website: http://www.IntegratedHealthHemp.com. The purpose of this site is really to bring into one place some of the top of the line hemp extracts / CBD products available on the market today.

I would like to talk about the difference between “broad spectrum” hemp extract and “full spectrum” hemp extract. Both of those extracts are derived from the industrial hemp plant. When you do the extracts, the nature of the extract of the industrial hemp plant is that is has a THC level of less than 0.3%. This is not enough to cause “highs” or any problems. However, from a drug testing point of view, even if there is 0.2% THC in a hemp extract and someone takes it every day, it builds up in the body, and you might fail a drug test.

The industry has defined two levels. One is called a broad spectrum hemp extract and one is called full spectrum hemp extract. In the case of the broad spectrum hemp extract, there is further processing of the product so that THC is totally removed. In the case of the full spectrum hemp extract, there is no further processing, so you have small amounts of THC along with all the other ingredients, the cannabinoids and terpenes. In fact, from a full spectrum hemp extract, you tend to get a more effective product, but then you are in the position of perhaps not passing a drug test.

As we go through discussions of the products, you will see there are products in which we have broad spectrum hemp extract and products that have the full spectrum hemp extract, so you have choices to make. Still, with extremely effective products.

I would like to give an overview of products we carry on the new website. We have seven products. Five of them we buy from a company called Quicksilver Scientific. The reason we do is that they have a very special technology for delivering ingredients into the body that is beyond the typical oral supplement. This is called a “liposomal delivery system” where you actually pack the ingredients into a #liposome, which is very small, and can actually be absorbed through the tissues of the mouth and upper GI tract to get into the body very quickly and efficiently.

One of the reasons we love these Quicksilver Scientific (QS) products so much is that you might be able to get, for example, up to seven times more into your system within the time period of a few minutes. When you can do that, because you can get so much more in effectively, you don’t have to use as much in the first place. And secondly, because it goes in so quickly, you begin to get the effects and the benefits almost immediately.

The first two products I’d like to talk about are #hemp extracts. One is called Nanoemulsified Broad Spectrum Hemp Extract 400 and one is called Nanoemulsified Full Spectrum Hemp Extract 400. The broad spectrum has 0% THC and the full spectrum has a small amount, less than 0.3% THC. But if you were tested, you would not test positive with the broad spectrum, and you may test positive with the full spectrum. You have a choice from that perspective.

The next group of products I want to talk about are products Quicksilver just introduced, and they are called the CBD Synergies products. One of the things I like about these CBD Synergies products is where you combine the effects of the CBD and hemp extract with synergistic ingredients that give you a broader range of applicability and effectiveness.

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Health Products Distributors, Inc. (HPDI) has helped people create health naturally since 1988. Our main website (http://www.IntegratedHealth.com) since 1997 is a major resource for individuals striving to live healthfully and provides information and products formulated to support optimal health. The new website for hemp extracts and CBD products is intended to complement the products on the main HPDI website.

HEMP EXTRACTS — RESOURCES

QUICKSILVER NANOEMULSIFIED FULL SPECTRUM HEMP EXTRACT 400
https://integratedhealthhemp.com/product/quicksilver-nanoemulsified-full-spectrum-hemp-extract-400/

QUICKSILVER NANOEMULSIFIED BROAD SPECTRUM HEMP EXTRACT 400
https://integratedhealthhemp.com/product/quicksilver-nanoemulsified-broad-spectrum-hemp-extract/

QUICKSILVER COLORADO NANOEMULSIFIED HEMP OIL (Discontinued)

OTHER QUICKSILVER HEMP/CBD PRODUCTS

CBD SYNERGIES-AX CALMING FORMULA
 https://integratedhealthhemp.com/product/cbd-synergies-ax-calming-formula/

CBD SYNERGIES PN-RELIEF PAIN FORMULA:
https://integratedhealthhemp.com/product/cbd-synergies-pn-relief-formula/

CBD SYNERGIES-SP SLEEP FORMULA:
https://integratedhealthhemp.com/product/cbd-synergies-sp-sleep-formula/

QTANEOUS PRODUCTS

SOOTHE & EASE TOPICAL SPRAY (with HEMP EXTRACT AND DMSO)
https://integratedhealthhemp.com/product-category/topical/

ALL HEMP EXTRACTS & CBD PRODUCTS: https://integratedhealthhemp.com/product-category/cbd-supplements/

RELATED ARTICLES

Recent Advances in Liposome Technology by Hank Liers, PhD

Benefits of Liposomal Nutrients (Liposomes) by Hank Liers, PhD

Quicksilver Liposomal Formulas – New Products by Fred Liers, PhD

Report from ACAM 2015 Annual Meeting by Fred Liers, PhD

RELATED HPDI YOUTUBE VIDEOS:

https://youtu.be/JbEnlcofBEA
“Introducing IntegratedHealthHemp.com for Hemp Extracts / CBD Supplements and Topicals”

https://youtu.be/JpU5VJX-k5U
“New! Nanoemulsified Broad Spectrum & Full Spectrum Hemp Extracts from Quicksilver Scientific”

https://youtu.be/XhIkZvCdjaw
“CBD Oil In Depth: Nanoemulsified Colorado Hemp Oil from Quicksilver Scientific.”

These videos feature Dr. Hank Liers, founder and chief formulator of Health Products Distributors, Inc. – HPDI

HEALTH PRODUCTS DISTRIBUTORS, INC. (since 1988)

http://www.INTEGRATEDHEALTH.COM (Retail)

http://www.HEALTHPRODUCTSDISTRIBUTORS.com (Reseller / Wholesale)

https://www.INTEGRATEDHEALTHHEMP.com (Hemp Extracts and CBD products)

 

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NEW NANOEMULSIFIED HEMP EXTRACTS FROM QUICKSILVER

Dr. Hank Liers, PhD nanoemulsifed hemp extracts Quicksilver Scientific CBDToday I talk about two new hemp extracts available from HPDI in the Quicksilver brand. I specifically present the difference between two new products: Nanoemulsified Broad Spectrum Hemp Extract 400 and Nanoemulsified Full Spectrum Hemp Extract 400. These two products replace the single product known as “Colorado Hemp Oil” previously available from Quicksilver. We now offer these two products and a range of hemp and CBD formulas on our new hemp website: www.INTEGRATEDHEALTHHEMP.COM.

Quicksilver Scientific replaces its Colorado Hemp Oil with two superb hemp extract formulas. The new formulas (Broad Spectrum and Full Spectrum) are similar. They both have the same level (21 mg) per serving of hemp extract. Also, they both contain 16 mg cannabidiol, which is an increase from the 12 mg cannabidiol in the old product (Colorado Hemp Oil).

In addition, both of the new hemp extract formulas contain more ingredients associated with hemp extracts—including additional cannabinoids, terpenes, and beta-caryophyllene—that improve the effectiveness of hemp extract products.

The main difference between the Broad Spectrum and the Full Spectrum hemp extract formulas is that the Broad Spectrum Hemp Extract has 0% THC, and the Full Spectrum Hemp Extract contains a very small amount (less than 0.3% THC).

For people tho are regular tested for drugs (e.g., THC), the Broad Spectrum Hemp Extract is recommended. For those individuals who are not drug tested, the Full Spectrum Hemp Extract is recommended because it will better support the functioning of the endocannabinoid system.

The price of the Nanoemusified Full Spectrum Hemp Extract 400 and the Nanoemusified Broad Spectrum Hemp Extract 400 hemp extract products remain the same as the price for the discontinued Nanoemulsified Colorado Hemp Oil product. HPDI currently sells each of these new hemp extract liposomal products for $78.50 (retail).

These two new Quicksilver Scientific hemp extract formulas are superior liposomal hemp extract products. Both Broad Spectrum Hemp Extract and Full Spectrum Hemp Extract are highly effective formulas utilizing unique nanoemulsification technology to maximize the many known benefits of hemp extract.

Broad Spectrum Full Spectrum Hemp Extracts

Broad Spectrum and Full Spectrum Hemp Extracts

HEMP EXTRACTS — RESOURCES

QUICKSILVER NANOEMULSIFIED FULL SPECTRUM HEMP EXTRACT 400
https://integratedhealthhemp.com/product/quicksilver-nanoemulsified-full-spectrum-hemp-extract-400/

QUICKSILVER NANOEMULSIFIED BROAD SPECTRUM HEMP EXTRACT 400
https://integratedhealthhemp.com/product/quicksilver-nanoemulsified-broad-spectrum-hemp-extract/

QUICKSILVER COLORADO NANOEMULSIFIED HEMP OIL (Discontinued)

OTHER QUICKSILVER HEMP/CBD PRODUCTS

CBD SYNERGIES-AX CALMING FORMULA
 https://integratedhealthhemp.com/product/cbd-synergies-ax-calming-formula/

CBD SYNERGIES PN-RELIEF PAIN FORMULA:
https://integratedhealthhemp.com/product/cbd-synergies-pn-relief-formula/

CBD SYNERGIES-SP SLEEP FORMULA:
https://integratedhealthhemp.com/product/cbd-synergies-sp-sleep-formula/

QTANEOUS PRODUCTS

SOOTHE & EASE TOPICAL SPRAY (with HEMP EXTRACT AND DMSO)
https://integratedhealthhemp.com/product-category/topical/

ALL HEMP EXTRACTS & CBD PRODUCTS: https://integratedhealthhemp.com/product-category/cbd-supplements/

Related HPDI YouTube videos:

https://youtu.be/JbEnlcofBEA
“Introducing IntegratedHealthHemp.com for Hemp Extracts / CBD Supplements and Topicals”

https://youtu.be/JpU5VJX-k5U
“New! Nanoemulsified Broad Spectrum & Full Spectrum Hemp Extracts from Quicksilver Scientific”

https://youtu.be/XhIkZvCdjaw
“CBD Oil In Depth: Nanoemulsified Colorado Hemp Oil from Quicksilver Scientific.”

These videos feature Dr. Hank Liers, founder and chief formulator of Health Products Distributors, Inc. – HPDI

Related HPDI Blog articles:

Introducing a New HPDI Website for Hemp Extracts and CBD Products: https://INTEGRATEDHEALTHHEMP.COM

CBD Oil In Depth: Nanoemulsified Colorado Hemp Oil from Quicksilver Scientific by Hank Liers, PhD
(http://www.healthproductsdistributors.com/blog/cbd-oil-in-depth-colorado-hemp-oil/)

Quicksilver Liposomal Formulas – New Products! by Fred Liers, PhD
(http://www.integratedhealthblog.com/quicksilver-liposomal-formulas/)

Recent Advances in Liposome Technology by Hank Liers, PhD
(http://www.integratedhealthblog.com/recent-advances-liposomes-technology/)

Quicksilver Broad Spectrum Hemp Extract 400

Quicksilver Broad Spectrum Hemp Extract

Full Spectrum Hemp Extract 400

Quicksilver Full Spectrum Hemp Extract

HPDI WEBSITES

HPDI website (retail): http://www.IntegratedHealth.com

HPDI hemp website: https://www.IntegratedHealthHemp.com

HPDI reseller website: http://www.HealthProductsDistributors.com

HPDI blog: http://www.IntegratedHealthBlog.com

 

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ULTIMATE PROTECTOR+ INGREDIENTS – GREEN TEA EXTRACT

Dr. Hank Liers, PhD biography about us HPDI integratedhealth formulator founder CEO scientist physicist wild bilberry and wild blueberry Ultimate Protector+ includes green tea extract, as well as extracts from 12 different fruits, vegetables, and herbs. Each of these ingredients contain substances that may be considered to be polyphenols, antioxidants, and Nrf2 activators. In this article, I explore the ingredient green tea extract. Green tea extract is added as a separate ingredient in Ultimate Protector+.

Ultimate Protector+ Includes Green Tea Extract

Ultimate Protector+ Includes Green Tea Extract

Green tea extract (high in EGCG) is obtained from the unfermented leaves of Camellia sinensis for which numerous biological activities have been reported including: antimutagenic, antibacterial, hypocholesterolemic, antioxidant, and protective against tumorigenesis.

The green tea extract in Ultimate Protector+ has been extracted with non-GMO food grade ethanol and distilled water. Testing has indicated the product over 93% polyphenols, over 50% EGCG, and about 4% caffeine.

Bioactive Compounds in Tea
Tea contains over 2,000 components, including polyphenols (flavonoids), pigments (carotenoids and chlorophyll), alkaloids (caffeine, theophylline, theobromine), lignans, carbohydrates, lipids, proteins, amino acids (including L-theanine), vitamins (vitamin C, vitamin E, riboflavin), and various minerals and trace elements.

Flavonoids in Green Tea
Dietary flavonoids are divided in six subclasses: flavan-3-ols, anthocyanidins, flavanones, flavonols, flavones, and isoflavones. Total flavonoid content in green tea is about 138 mg a per 100 mL. A major subclass of flavonoids in green tea is that of flavan-3-ols. Flavan-3-ol monomers, also known as catechins, constitute 30%-42% of the solid weight of brewed green tea. The principal catechins found in tea are (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), and (-)-epigallocatechin gallate (EGCG).

EGCG (Epigallocatechin Gallate) is one of the most powerful compounds in green tea. It has been studied to treat various diseases and may be one of the main reasons green tea has such powerful medicinal properties

Vitamins and Minerals Found in Green Tea

Green Tea Plant

GREEN TEA STUDIES

Below we provide information from several research articles that highlight some of the potential health effects of green tea extracts.

1. EGCG upregulates phase-2 detoxifying and antioxidant enzymes via the Nrf2 signaling pathway in human breast epithelial cells

Hye-Kyung Na and Young-Joon Surh
Proc Amer Assoc Cancer Res, Volume 46, 2005
From: http://cancerres.aacrjournals.org/content/65/9_Supplement/367.1

Abstract

There are multiple lines of compelling evidence from epidemiologic and laboratory studies supporting that frequent consumption of green tea is inversely associated with the risk of several types of human cancer and other chronic diseases. The chemopreventive and chemoprotective activity of green tea have been attributed to the polyphenolic ingredient (-)epigallocatechin-3-gallate (EGCG). Oxidative DNA damage has been implicated in initiation of carcinogenesis. The induction of phase-2 detoxifying or antioxidant defense enzymes contributes to the cancer chemopreventive and cytoprotective effects of many phytochemicals against oxidative stress. Here, we report that treatment of human breast epithelial (MCF10A) cells with EGCG induces the mRNA expression of both modulatory and catalytic subunits of γ-glutamate-cystein ligase (GCL), which is the rate-limiting enzyme in the synthesis of GSH. In addition, EGCG upregulated the expression of other antioxidant enzymes, including manganese superoxide dismutase and glutathione S-transferase π in a concentration- and time-dependent manner. NF-E2-related factor (Nrf2), a basic-leucine zipper transcription factor, has been reported to regulate the antioxidant/electrophile responsive elements (ARE/EpRE)-mediated expression of various phase-2 detoxifying or antioxidant enzymes. The nuclear accumulation and ARE/EpRE binding of Nrf2 were increased in EGCG-treated MCF10A cells. Moreover, MCF10A cells transfected with the luciferase reporter gene under the control of ARE/EpRE-driven promoter exhibited a strong transcriptional activity following exposure to EGCG. In contrast, mutation of the GC core box in the ARE/EpRE-driven promoter abolished the EGCG-induced transcriptional activity. MCF10A cells transiently transfected with dominant negative Nrf-2 were less responsive to EGCG-induced expression of GCLC mRNA compared to the vector-transfected control cells. Furthermore, EGCG treatment activated the extracellular signal-regulated protein kinase1/2 and Akt through phosphorylation. These findings, taken together, suggest that induction of antioxidant enzymes by EGCG can be mediated by activation of Nrf2 and possibly upstream signaling kinases, which may provide the cells with acquired antioxidant defense capacity against oxidative insult.

2. Neurological mechanisms of green tea polyphenols in Alzheimer’s and Parkinson’s diseases

The Journal of Nutritional Biochemistry, Volume 15, Issue 9, September 2004, Pages 506-516
OrlyWeinreba, SilviaMandela, TamarAmitaMoussa, B.H.Youdima
From: https://doi.org/10.1016/j.jnutbio.2004.05.002

Abstract
Tea consumption is varying its status from a mere ancient beverage and a lifestyle habit, to a nutrient endowed with possible prospective neurobiological–pharmacological actions beneficial to human health. Accumulating evidence suggest that oxidative stress resulting in reactive oxygen species generation and inflammation play a pivotal role in neurodegenerative diseases, supporting the implementation of radical scavengers, transition metal (e.g., iron and copper) chelators, and nonvitamin natural antioxidant polyphenols in the clinic. These observations are in line with the current view that polyphenolic dietary supplementation may have an impact on cognitive deficits in individuals of advanced age. As a consequence, green tea polyphenols are now being considered as therapeutic agents in well controlled epidemiological studies, aimed to alter brain aging processes and to serve as possible neuroprotective agents in progressive neurodegenerative disorders such as Parkinson’s and Alzheimer’s diseases. In particular, literature on the putative novel neuroprotective mechanism of the major green tea polyphenol, (−)-epigallocatechin-3-gallate (EGCG), are examined and discussed in this review.

3. Renoprotective effects of (+)-catechin in streptozotocin-induced diabetic rat model

Abstract
Diabetic nephropathy is a complication of diabetes mellitus leading to end-stage renal disease. Oxidative stress and inflammation play a major role in the pathogenesis of diabetic nephropathy. Green tea, known for its antioxidant and anti-inflammatory properties, has been shown to be renoprotective. We hypothesized that (+)-catechin (CTN), a component of green tea, is responsible for the renoprotection. Our investigation of the therapeutic potential of CTN in streptozotocin-induced diabetic rats demonstrated for the first time that the effects of CTN treatment were comparable with the effects of an angiotensin-converting enzyme inhibitor (ACEi) enalapril for the treatment of albumin excretion. After 12 weeks of CTN treatment with 35 mg/d in the drinking water, urinary albumin excretion and plasma creatinine concentrations in all the diabetic treatment groups were reduced, compared with the diabetic group with no treatment. Urine creatinine and creatinine clearance were higher in diabetic groups treated with CTN and ACEi compared with the diabetic group with no treatment. Endothelin 1, lipid peroxidation, concentration of alanine transferase enzyme, and expression of fibronectin were lower in all the treatment groups compared with the diabetic group with no treatment. Concentrations of free thiols were higher in the CTN-treated group compared with the diabetic rats with no treatment. Our findings suggest that CTN has renoprotective properties comparable with ACEi, and coadministration of CTN and enalapril might be useful in reducing albumin excretion as well as improving endothelial function. (+)-Catechin might be successfully used in the future for clinical situations where ACEi is poorly tolerated or contraindicated.

4. Green Tea Polyphenol (−)-Epigallocatechin-3-Gallate Restores Nrf2 Activity and Ameliorates Crescentic Glomerulonephritis

published: March 18, 2015https://doi.org/10.1371/journal.pone.0119543
Ting Ye , Junhui Zhen , Yong Du , Jason K. Zhou, Ai Peng, Nosratola D. Vaziri, Chandra Mohan , Yan Xu , Xin J. Zhou
From: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0119543

Abstract
Crescentic glomerulonephritis (GN) is the most severe form of GN and is associated with significant morbidity and mortality despite aggressive immunotherapy with steroids, cytotoxic drugs, and plasmapheresis. We examined the therapeutic efficacy of the green tea polyphenol (−)-epigallocatechin-3-gallate (EGCG, 50 mg/kg BW/day x3weeks), a potent anti-inflammatory and anti-oxidant agent, on experimental crescentic GN induced in 129/svJ mice by administration of rabbit anti-mouse glomerular basement membrane sera. Routine histology and key molecules involved in inflammatory and redox signaling were studied. EGCG treatment significantly reduced mortality, decreased proteinuria and serum creatinine, and markedly improved renal histology when compared with vehicle-treated mice. The improvements in renal function and histology were accompanied by the restoration of Nrf2 signaling (which was impaired in vehicle-treated mice) as shown by increased nuclear translocation of Nrf2 and cytoplasmic glutamate cysteine ligase catalytic subunit, glutamate cysteine ligase modifier subunit, and glutathione peroxidase. EGCG-treated mice also showed reduction in p-Akt, p-JNK, p-ERK1/2 and p-P38 as well as restoration of PPARγ and SIRT1 levels. Lower dose of EGCG (25 mg/kg BW/day x2 weeks) treatment also significantly decreased proteinuria and serum creatinine, and markedly improved renal histology when compared with vehicle-treated mice. Thus, our data illustrate the efficacy of EGCG in reversing the progression of crescentic GN in mice by targeting multiple signaling and inflammatory pathways as well as countering oxidative stress.

SUMMARY

Green Tea Extract is an exciting natural ingredient full of important polyphenols, catechins, antioxidants, and Nrf2 activators that help to make Ultimate Protector such an outstanding nutritional supplement. Continued research shows an amazing list of health benefits for this substance including its ability to function as a powerful stimulator of Nrf2 activity. It truly belongs in the Ultimate Protector+™ formula.

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ULTIMATE PROTECTOR+ INGREDIENTS – WHOLE GRAPE EXTRACT

Dr. Hank Liers, PhD biography about us HPDI integratedhealth formulator founder CEO scientist physicist wild bilberry and wild blueberryUltimate Protector+ contains whole grape extract, as well as components from 12 different fruits, vegetables, and herbs. Each of these ingredients contain substances that may be considered to be polyphenols, antioxidants, and Nrf2 activators. In this article, I explore the ingredient whole grape extract (including seeds, pulp, and skin), which is a component of SFB® – Standardized Fruit Blend and VinCare® from Ethical Naturals, Inc.

Ultimate Protector+ Includes Whole Grape Extract

Ultimate Protector+ Includes Whole Grape Extract

SFB® – Standardized Fruit Blend

SFB® Standardized Fruit Blend is a proprietary formula that combines extracts from Grape, Cranberry, Pomegranate, Blueberry, Apple, Mangosteen, Bilberry, Chokeberry, and Goji Berry. High in fruit polyphenols, anthocyanidins, proanthocyanidins, catechins, ellagic acid, chlorogenic acid, resveratrol, and quinic acid. With its diverse blend, SFB® offers 40-50% polyphenols as well as >9,000 ORAC units in a single gram.

Polyphenols, anthocyanidins, and other known plant components are powerful ingredients associated with a variety of areas of human health, including healthy aging, healthy glucose metabolism, cardiovascular health, and inflammation management.

VinCare® – Standardized Whole Grape Extract

VinCare® is a whole grape extract from the seeds, skin, and pulp of red grapes. Whole Grape Extract contains highly bioavailable bioflavonoid complexes that in research studies have been shown to have powerful antioxidant capability. The Oligomeric Proanthocyanidins (OPCs) in grape extract are able to strengthen collagen fibers in aging or damaged connective tissue and can act as a preventative against connective tissue degradation. Some research indicates that anthocyanidins, which are found in extracts of grape seed, skin, and pulp (but not in grape seed extract), can reduce oxidized glutathione while at the same time become reduced themselves. In addition, extracts of grape skin and pulp (but not those of grape seed extract) contain trans-resveratrol that has been shown to have cell protective effects.

Grape seed extract has been reported to demonstrate a remarkable spectrum of biological, pharmacological and therapeutic properties against oxidative stress. The antioxidative activities of grape seed extract have been found to be much stronger than those of vitamins C and E. Studies have indicated that grape seed extract showed a protective effect on cardiovascular disease, nephropathy, atherosclerosis, and neuropathy, among other conditions.

Vincare® contains ~80% polypnenols and has an ORAC value of about 19,000 µmole TE/g. ORAC 5.0 testing of grape seed extract exhibits one of the highest values of any tested material at about 100,000 µmole TE/g.

It has been shown that grape seed OPCs activate nuclear erythroid2-related factor2 (Nrf2), which is a key antioxidative transcription factor, with the concomitant elevation of downstream hemeoxygenase-1 (HO-1).

grape extract

HISTORY OF HEALTH PRODUCTS DISTRIBUTORS USE OF WHOLE GRAPE AND GRAPE SEED EXTRACTS

During the last 24 years I have designed more than 20 products incorporating OPCs from whole grape and grape seed extracts. Some of these products include: Antioxidant Formula, Diabetes Support Formula, Eye & Vision Formula, Joint Health FormulaChewable Kid’s Mighty-MultiHank & Brian’s Mighty Multi-vite!Mini MultiMulti Two, OPC-C, PRO-CProlytRejuvenate! Pro, and Rejuvenate! Berries & Herbs.

In 1993 I prepared an extensive review of OPCs including the sources of grape seed extract and pine bark extract. In this review article entitled Review of Scientific Research on Oligomeric Proanthcyanidins (OPC), I pointed out that grape seed extract consists of approximately 92% polyphenols, 32% monomers (flavan-3-ol), and 68% OPCs. OPCs consist of  catechins (referring to both catechins and epicatechins) that have the peculiar property of forming polymers with themselves. When the number of connected catechins is 10 or less they are called oligomers and thus the term used is “oligomeric proanthocyanidins.” When the number of connected catechins is more than 10 the term condensed tannins is generally used. The term proanthocyanidins comes about because when these materials are subjected to 10% hydrochloric acid and heated to boiling (this is what is termed the Bate-Smith test), they yield an anthocyanidin, with its intense red coloration, and a catechin.

grape and grape seeds

Below we provide information from several research articles that highlight some of the potential health effects of whole grape and grape seed extracts.

Procyanidins from Wild Grape (Vitis amurensis) Seeds Regulate ARE-Mediated Enzyme Expression via Nrf2 Coupled with p38 and PI3K/Akt Pathway in HepG2 Cells

From: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269721/

Abstract

Procyanidins, polymers of flavan-3-ol units, have been reported to exhibit many beneficial health effects such as antioxidant and anti-carcinogenic effects. In this study, we investigated the cancer chemopreventive properties of procyanidins from wild grape (Vitis amurensis) seeds in particular their roles in inducing phase II detoxifying/antioxidant enzymes as well as in modulating the upstream kinases. Ethanolic extract of V. amurensis seeds was fractionated with a series of organic solvents and finally separated into six fractions, F1–F6. Chemical properties of the procyanidins were analyzed by vanillin assay, BuOH-HCl test, and depolymerization with phloroglucinol followed by LC/MS analysis. The F5 had the highest procyanidin content among all the fractions and strongly induced the reporter activity of antioxidant response element as well as the protein expression of nuclear factor E2-related factor (Nrf2) in HepG2 human hepatocarcinoma cells. The procyanidin-rich F5 also strongly induced the expression of the phase II detoxifying and antioxidant enzymes such as NAD(P)H:quinone oxidoreductase1 and hemeoxygenase1. Phosphorylations of the upstream kinases such as MAPKs and PI3K/Akt were significantly increased by treatment with procyanidin fraction. In addition, the procyanidin-mediated Nrf2 expression was partly attenuated by PI3K inhibitor LY294002, and almost completely by p38 inhibitor SB202190, but neither by JNK inhibitor SP600125 nor by MEK1/2 inhibitor U0126. Taken together, the procyanidins from wild grape seeds could be used as a potential natural chemopreventive agent through Nrf2/ARE-mediated phase II detoxifying/antioxidant enzymes induction via p38 and PI3K/Akt pathway.

Keywords: wild grape seed, Vitis amurensis, procyanidin, chemoprevention, MAPKs, Nrf2, phase II detoxifying enzyme, antioxidant enzyme

 

Grape seed extract induces apoptotic death of human prostate carcinoma DU145 cells via caspases activation accompanied by dissipation of mitochondrial membrane potential and cytochrome c release.

From: http://www.ncbi.nlm.nih.gov/pubmed/12419835

Carcinogenesis. 2002 Nov;23(11):1869-76, Agarwal C1, Singh RPAgarwal R.

Abstract

Grape seed extract (GSE), rich in the bioflavonoids commonly known as procyanidins, is one of the most commonly consumed dietary supplements in the United States because of its several health benefits. Epidemiological studies show that many prostate cancer (PCA) patients use herbal extracts as dietary supplements in addition to their prescription drugs. Accordingly, in recent years, we have focused our attention on assessing the efficacy of GSE against PCA. Our studies showed that GSE inhibits growth and induces apoptotic death of human PCA cells in culture and in nude mice. Here, we performed detailed studies to define the molecular mechanism of GSE-induced apoptosis in advanced human PCA DU145 cells. GSE treatment of cells at various doses (50-200 micro g/ml) for 12-72 h resulted in a moderate to strong apoptotic death in a dose- and time-dependent manner. In the studies assessing the apoptotic-signaling pathway induced by GSE, we observed an increase in cleaved fragments of caspases 3, 7 and 9 as well as PARP in GSE-treated cells after 48 and 72 h of treatment. Pre-treatment of cells with general caspases inhibitor, z-Val-Ala-Asp(OMe)-FMK or caspase 3-like proteases inhibitor [z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-FMK], almost completely (approximately 90%) inhibited the GSE-induced apoptotic cell death. In a later case, GSE-induced caspase-3 activity was completely inhibited. Selective caspase 9 inhibitor [z-Leu-Glu(OMe)-His-Asp(OMe)-FMK] showed only partial inhibition of GSE-induced apoptosis whereas GSE-induced protease activity of caspase 9 was completely inhibited. Upstream of caspase cascade, GSE showed disappearance of mitochondrial membrane potential and an increase in cytochrome c release in cytosol. Together, these results suggest that GSE possibly causes mitochondrial damage leading to cytochrome c release in cytosol and activation of caspases resulting in PARP cleavage and execution of apoptotic death of human PCA DU145 cells. Furthermore, GSE-caused caspase 3-mediated apoptosis also involves other pathway(s) including caspase 9 activation.

Differential effect of grape seed extract against human non-small-cell lung cancer cells: the role of reactive oxygen species and apoptosis induction.

From: http://www.ncbi.nlm.nih.gov/pubmed/23682782

Abstract

The present study examines grape seed extract (GSE) efficacy against a series of non-small-cell lung cancer (NSCLC) cell lines that differ in their Kras and p53 status to establish GSE potential as a cytotoxic agent against a wide range of lung cancer cells. GSE suppressed growth and induced apoptotic death in NSCLC cells irrespective of their k-Ras status, with more sensitivity toward H460 and H322 (wt k-Ras) than A549 and H1299 cells (mutated k-Ras). Mechanistic studies in A549 and H460 cells, selected, based on comparative efficacy of GSE at higher and lower doses, respectively, showed that apoptotic death involves cytochrome c release associated caspases 9 and 3 activation, and poly (ADP-ribosyl) polymerase cleavage, strong phosphorylation of ERK1/2 and JNK1/2, downregulation of cell survival proteins, and upregulated proapoptotic Bak expression. Importantly, GSE treatment caused a strong superoxide radical-associated oxidative stress, significantly decreased intracellular reduced glutathione levels, suggesting, for the first time, the involvement of GSE-caused oxidative stress in its apoptotic inducing activity in these cells. Because GSE is a widely-consumed dietary agent with no known untoward effects, our results support future studies to establish GSE efficacy and usefulness against NSCLC control.

Role of oxidative stress in cytotoxicity of grape seed extract in human bladder cancer cells.

From: http://www.ncbi.nlm.nih.gov/pubmed/23831192

Food Chem Toxicol. 2013 Nov;61:187-95. doi: 10.1016/j.fct.2013.06.039. Epub 2013 Jul 3. Raina K1, Tyagi AKumar DAgarwal RAgarwal C.

Abstract

In present study, we evaluated grape seed extract (GSE) efficacy against bladder cancer and associated mechanism in two different bladder cancercell lines T24 and HTB9. A significant inhibitory effect of GSE on cancer cell viability was observed, which was due to apoptotic cell death. Cell death events were preceded by vacuolar appearance in cytoplasm, which under electron microscopy was confirmed as swollen mitochondrial organelle and autophagosomes. Through detailed in vitro studies, we established that GSE generated oxidative stress that initiated an apoptotic response as indicated by the reversal of GSE-mediated apoptosis when the cells were pre-treated with antioxidants prior to GSE. However, parallel to a strong apoptotic cell death event, GSE also caused a pro-survival autophagic event as evidenced by tracking the dynamics of LC3-II within the cells. Since the pro-death apoptotic response was stronger than the pro-survival autophagy induction within the cells, cell eventually succumbed to cellular death after GSE exposure. Together, the findings in the present study are both novel and highly significant in establishing, for the first time, that GSE-mediated oxidative stress causes a strong programmed cell death in human bladder cancer cells, suggesting and advocating the effectiveness of this non-toxic agent against this deadly malignancy.

Copyright © 2013 Elsevier Ltd. All rights reserved.

Target identification of grape seed extract in colorectal cancer using drug affinity responsive target stability (DARTS) technique: role of endoplasmic reticulum stress response proteins.

From: http://www.ncbi.nlm.nih.gov/pubmed/24724981

Abstract

Various natural agents, including grape seed extract (GSE), have shown considerable chemopreventive and anti-cancer efficacy against different cancers in pre-clinical studies; however, their specific protein targets are largely unknown and thus, their clinical usefulness is marred by limited scientific evidences about their direct cellular targets. Accordingly, herein, employing, for the first time, the recently developed drug affinity responsive target stability (DARTS) technique, we aimed to profile the potential protein targets of GSE in human colorectal cancer (CRC) cells. Unlike other methods, which can cause chemical alteration of the drug components to allow for detection, this approach relies on the fact that a drug bound protein may become less susceptible to proteolysis and hence the enriched proteins can be detected by Mass Spectroscopy methods. Our results, utilizing the DARTS technique followed by examination of the spectral output by LC/MS and the MASCOT data, revealed that GSE targets endoplasmic reticulum (ER) stress response proteins resulting in overall down regulation of proteins involved in translation and that GSE also causes oxidative protein modifications, specifically on methionine amino acids residues on its protein targets. Corroborating these findings, mechanistic studies revealed that GSE indeed caused ER stress and strongly inhibited PI3k-Akt-mTOR pathway for its biological effects in CRC cells. Furthermore, bioenergetics studies indicated that GSE also interferes with glycolysis and mitochondrial metabolism in CRC cells. Together, the present study identifying GSE molecular targets in CRC cells, combined with its efficacy in vast pre-clinical CRC models, further supports its usefulness for CRC prevention and treatment.

Polyphenolics in grape seeds-biochemistry and functionality.

From: http://www.ncbi.nlm.nih.gov/pubmed/14977436

J Med Food. 2003 Winter;6(4):291-9.

Abstract

Grape seeds are waste products of the winery and grape juice industry. These seeds contain lipid, protein, carbohydrates, and 5-8% polyphenols depending on the variety. Polyphenols in grape seeds are mainly flavonoids, including gallic acid, the monomeric flavan-3-ols catechin, epicatechin, gallocatechin, epigallocatechin, and epicatechin 3-O-gallate, and procyanidin dimers, trimers, and more highly polymerized procyanidins. Grape seed extract is known as a powerful antioxidant that protects the body from premature aging, disease, and decay. Grape seeds contains mainly phenols such as proanthocyanidins (oligomeric proanthocyanidins). Scientific studies have shown that the antioxidant power of proanthocyanidins is 20 times greater than vitamin E and 50 times greater than vitamin C. Extensive research suggests that grape seed extract is beneficial in many areas of health because of its antioxidant effect to bond with collagen, promoting youthful skin, cell health, elasticity, and flexibility. Other studies have shown that proanthocyanidins help to protect the body from sun damage, to improve vision, to improve flexibility in joints, arteries, and body tissues such as the heart, and to improve blood circulation by strengthening capillaries, arteries, and veins. The most abundant phenolic compounds isolated from grapeseed are catechins, epicatechin, procyanidin, and some dimers and trimers.

Anti-tumor-promoting activity of a polyphenolic fraction isolated from grape seeds in the mouse skin two-stage initiation-promotion protocol and identification of procyanidin B5-3′-gallate as the most effective antioxidant constituent.

From: http://www.ncbi.nlm.nih.gov/pubmed/10469619

Abstract

Procyanidins present in grape seeds are known to exert anti-inflammatory, anti-arthritic and anti-allergic activities, prevent skin aging, scavenge oxygen free radicals and inhibit UV radiation-induced peroxidation activity. Since most of these events are associated with the tumor promotion stage of carcinogenesis, these studies suggest that grape seed polyphenols and the procyanidins present therein could be anticarcinogenic and/or anti-tumor-promoting agents. Therefore, we assessed the anti-tumor-promoting effect of a polyphenolic fraction isolated from grape seeds (GSP) employing the 7,12-dimethylbenz[a]anthracene (DMBA)-initiated and 12-O-tetradecanoylphorbol 13-acetate (TPA)-promoted SENCAR mouse skin two-stage carcinogenesis protocol as a model system. Following tumor initiation with DMBA, topical application of GSP at doses of 0.5 and 1.5 mg/mouse/application to the dorsal initiated mouse skin resulted in a highly significant inhibition of TPA tumor promotion. The observed anti-tumor-promoting effects of GSP were dose dependent and were evident in terms of a reduction in tumor incidence (35 and 60% inhibition), tumor multiplicity (61 and 83% inhibition) and tumor volume (67 and 87% inhibition) at both 0.5 and 1.5 mg GSP, respectively. Based on these results, we directed our efforts to separate and identify the individual polyphenols present in GSP and assess their antioxidant activity in terms of inhibition of epidermal lipid peroxidation. Employing HPLC followed by comparison with authentic standards for retention times in HPLC profiles, physiochemical properties and spectral analysis, nine individual polyphenols were identified as catechin, epicatechin, procyanidins B1-B5 and C1 and procyanidin B5-3′-gallate. Five of these individual polyphenols with evident structural differences, namely catechin, procyanidin B2, procyanidin B5, procyanidin C1 and procyanidin B5-3′-gallate, were assessed for antioxidant activity. All of them significantly inhibited epidermal lipid peroxidation, albeit to different levels. A structure-activity relationship study showed that with an increase in the degree of polymerization in polyphenol structure, the inhibitory potential towards lipid peroxidation increased. In addition, the position of linkage between inter-flavan units also influences lipid peroxidation activity; procyanidin isomers with a 4-6 linkage showed stronger inhibitory activity than isomers with a 4-8 linkage. A sharp increase in the inhibition of epidermal lipid peroxidation was also evident when a gallate group was linked at the 3′-hydroxy position of a procyanidin dimer. Procyanidin B5-3′-gallate showed the most potent antioxidant activity with an IC(50) of 20 microM in an epidermal lipid peroxidation assay. Taken together, for the first time these results show that grape seed polyphenols possess high anti-tumor-promoting activity due to the strong antioxidant effect of procyanidins present therein. In summary, grape seed polyphenols in general, and procyanidin B5-3′-gallate in particular, should be studied in more detail to be developed as cancer chemopreventive and/or anticarcinogenic agents.

SUMMARY

Whole Grape and Grape Seed Extracts (GSE) is an exciting natural ingredient full of important polyphenols, anthocyanidins, oligomeric proanthocyanidins (OPCs), antioxidants and Nrf2 activators that help to make Ultimate Protector+ such an outstanding nutritional supplement. This ingredient has been used extensively in nutritional supplement formulations for almost 25 years now. Continued research shows an amazing list of health benefits for this substance including its ability to function as a powerful stimulator of Nrf2 activity. It truly belongs in the Ultimate Protector+™ formula.

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ULTIMATE PROTECTOR+ INGREDIENTS – CRANBERRY

Dr. Hank Liers, PhD biography about us HPDI integratedhealth formulator founder CEO scientist physicist wild bilberry and wild blueberryUltimate Protector+ contains cranberry extract, as well as components from 12 different fruits, vegetables, and herbs. Each of these ingredients contain substances that may be considered to be polyphenols, antioxidants, and Nrf2 activators. In this article I will explore the ingredient cranberry, which is a component of SFB® – Standardized Fruit Blend from Ethical Naturals, Inc.

Ultimate Protector+ Includes Cranberry

Ultimate Protector+ Includes Cranberry Extract

SFB® – Standardized Fruit Blend

SFB® is a proprietary formula that combines extracts from Grape, Cranberry, Pomegranate, Blueberry, Apple, Mangosteen, Bilberry, Chokeberry, and Goji Berry. It is high in fruit polyphenols, flavonoids, anthocyanins, catechins, proanthocyanins, ellagic acid, xanthines, chlorogenic acid, pterostilbenes, resveratrol, phloridzin, quercetin, zeaxanthin, carotenoids, polysaccharides, quinic acid, and more. With its diverse blend, SFB® offers over 40-50% polyphenols as well as >9,000 ORAC units in a single gram.

Polyphenols, anthocyanins and other plant elements are powerful ingredients associated with a variety of areas of human health, including healthy aging, healthy glucose metabolism, cardiovascular health, and inflammation management.

HEALTH BENEFITS OF CRANBERRIES

Cranberries (Vaccinium macrocarpon) are native to the boggy regions of temperate and subalpine North America and Europe. Although Native Americans used them extensively, they were first cultivated in the U.S. in the early 19th century. Cranberries grow on viney plants belonging to the heath family Ericaceae that also includes blueberries, bilberries, huckleberries, and bearberries (Arctostaphylos uva ursi). Cranberries contain tannins, fiber, anthocyanins (and other flavonoids), and Vitamin C. Their tannins prevent bacteria from attaching to cells. Consequently, cranberries have been used against infections, including urinary tract infections. In addition, cranberries may be helpful in protecting against heart disease and stroke.

Cranberry extract is an especially good source of antioxidant polyphenols. In animal studies, the polyphenols in cranberries have been found to decrease levels of total cholesterol and so-called “bad” cholesterol. Cranberries may also inhibit the growth of tumors in human breast tissue and lower the risk of both stomach ulcers and gum disease. 

Here is a list of the antioxidant and anti-inflammatory phytonutrients in found in cranberry extract.

Type of Phytonutrient             Specific Molecules
Phenolic Acids                             hydroxybenzoic acids including vanillic acids;
—Phenolic Acids (cont.)             hydroxycinnamic acids inculding caffeic,
—Phenolic Acids (cont.)             coumaric, cinnamic, and ferulic acid
Proanthocyanidins                     epicatechins
Anthocyanins                              cyanidins, malvidins, and peonidins
Flavonoids                                   quercetin, myricetin, kaempferol
Triterpenoids                              ursolic acid

OTHER CRANBERRY INFORMATION

    • Cranberries hold significantly high amounts of phenolic flavonoid phytochemicals called oligomeric proanthocyanidins (OPC’s). Scientific studies have shown that consumption of the berries have potential health benefits against cancer, aging and neurological diseases, inflammation, diabetes, and bacterial infections.
    • Antioxidant compounds in cranberry extract including OPC’s, anthocyanidin flavonoids, cyanidin, peonidin and quercetin may prevent cardiovascular disease by counteracting against cholesterol plaque formation in the heart and blood vessels. Further, these compounds help the human body lower LDL cholesterol levels and increase HDL-good cholesterol levels in the blood.
    • Scientific studies show that cranberry juice consumption offers protection against gram-negative bacterial infections such as E.coli in the urinary system by inhibiting bacterial-attachment to the bladder and urethra.
    • It is known that cranberries turns urine acidic. This, together with the inhibition of bacterial adhesion helps prevent the formation of alkaline (calcium ammonium phosphate) stones in the urinary tract by working against proteus bacterial-infections.
    • In addition, the berries prevent plaque formation on the tooth enamel by interfering with the ability of the gram-negative bacterium, Streptococcus mutans, to stick to the surface. In this way cranberries helps prevent the development of cavities.
    • The berries are also good source of many vitamins like vitamin C, vitamin A, ß-carotene, lutein, zea-xanthin, and folate and minerals like potassium, and manganese.
  • Oxygen Radical Absorbance Capacity (ORAC) demonstrates cranberry at an ORAC score of 9584 µmol TE units per 100 g, one of the highest in the category of edible berries.

For more information on cranberries visit: http://www.whfoods.com/genpage.php?tname=foodspice&dbid=145

SCIENTIFIC STUDIES ON THE ANTIOXIDANT EFFECTS OF CRANBERRIES

Below, I provide relevant scientific studies on the antioxidant effects and potential health benefits of cranberries.

PREVENTION OF OXIDATIVE STRESS, INFLAMMATION AND MITOCHONDRIAL DYSFUNCTION IN THE INTESTINE BY DIFFERENT CRANBERRY PHENOLIC FRACTIONS.

ABSTRACT

Cranberry fruit has been reported to have high antioxidant effectiveness that is potentially linked to its richness in diversified polyphenolic content. The aim of the present study was to determine the role of cranberry polyphenolic fractions in oxidative stress (OxS), inflammation and mitochondrial functions using intestinal Caco-2/15 cells. The combination of HPLC and UltraPerformance LC®-tandem quadrupole (UPLC-TQD) techniques allowed us to characterize the profile of low, medium and high molecular mass polyphenolic compounds in cranberry extracts. The medium molecular mass fraction was enriched with flavonoids and procyanidin dimers whereas procyanidin oligomers (DP > 4) were the dominant class of polyphenols in the high molecular mass fraction. Pre-incubation of Caco-2/15 cells with these cranberry extracts prevented iron/ascorbate-mediated lipid peroxidation and counteracted lipopolysaccharide-mediated inflammation as evidenced by the decrease in pro-inflammatory cytokines (TNF-α and interleukin-6), cyclo-oxygenase-2 and prostaglandin E2. Cranberry polyphenols (CP) fractions limited both nuclear factor κB activation and Nrf2 down-regulation. Consistently, cranberry procyanidins alleviated OxS-dependent mitochondrial dysfunctions as shown by the rise in ATP production and the up-regulation of Bcl-2, as well as the decline of protein expression of cytochrome c and apoptotic-inducing factor. These mitochondrial effects were associated with a significant stimulation of peroxisome-proliferator-activated receptor γ co-activator-1-α, a central inducing factor of mitochondrial biogenesis and transcriptional co-activator of numerous downstream mediators. Finally, cranberry procyanidins forestalled the effect of iron/ascorbate on the protein expression of mitochondrial transcription factors (mtTFA, mtTFB1, mtTFB2). Our findings provide evidence for the capacity of CP to reduce intestinal OxS and inflammation while improving mitochondrial dysfunction.

 CHEMICAL CHARACTERIZATION AND CHEMO-PROTECTIVE ACTIVITY OF CRANBERRY PHENOLIC POWDERS IN A MODEL CELL CULTURE. RESPONSE OF THE ANTIOXIDANT DEFENSES AND REGULATION OF SIGNALING PATHWAYS

ABSTRACT

Oxidative stress and reactive oxygen species (ROS)-mediated cell damage are implicated in various chronic pathologies. Emerging studies show that polyphenols may act by increasing endogenous antioxidant defense potential. Cranberry has one of the highest polyphenol content among commonly consumed fruits. In this study, the hepato-protective activity of a cranberry juice (CJ) and cranberry extract (CE) powders against oxidative stress was screened using HepG2 cells, looking at ROS production, intracellular non-enzymatic and enzymatic antioxidant defenses by reduced glutathione concentration (GSH), glutathione peroxidase (GPx) and glutathione reductase (GR) activity and lipid peroxidation biomarker malondialdehyde (MDA). Involvement of major protein kinase signaling pathways was also evaluated. Both powders in basal conditions did not affect cell viability but decreased ROS production and increased GPx activity, conditions that may place the cells in favorable conditions against oxidative stress. Powder pre-treatment of HepG2 cells for 20 h significantly reduced cell damage induced by 400 μM tert-butylhydroperoxide (t-BOOH) for 2 h. Both powders (5–50 μg/ml) reduced t-BOOH-induced increase of MDA by 20% (CJ) and 25% (CE), and significantly reduced over-activated GPx and GR. CE, with a significantly higher amount of polyphenols than CJ, prevented a reduction in GSH and significantly reduced ROS production. CJ reversed the t-BOOH-induced increase in phospho-c-Jun N-terminal kinase. This study demonstrates that cranberry polyphenols may help protect liver cells against oxidative insult by modulating GSH concentration, ROS and MDA generation, antioxidant enzyme activity and cell signaling pathways.

CRANBERRY EXTRACT SUPPRESSES INTERLEUKIN-8 SECRETION FROM STOMACH CELLS STIMULATED BY HELICOBACTER PYLORI IN EVERY CLINICALLY SEPARATED STRAIN BUT INHIBITS GROWTH IN PART OF THE STRAINS

From: http://www.sciencedirect.com/science/article/pii/S1756464613000364

ABSTRACT

It is known that cranberry inhibits the growth of Helicobacter pylori (HP). In human stomach, HP basically induces chronic inflammation by stimulating stomach cells to secrete interleukin (IL)-8 and other inflammatory cytokines, and causes stomach cancer, etc. The aim of this study was to investigate the inhibiting effects of cranberry on HP growth and IL-8 secretion from stomach cells induced by HP, using clinically separated HP strains. HP growth in liquid culture and on-plate culture was evaluated by titration after 2-day incubation and by agar dilution technique, respectively. For IL-8 experiments, MKN-45, a stomach cancer cell line, was incubated with HP for 24 h and IL-8 in the medium was assayed by ELISA. Cranberry suppressed growth of the bacteria only in six of the 27 strains. Meanwhile, it suppressed IL-8 secretion in all the strains. The results may suggest a possible role of cranberry in prevention of stomach cancer by reducing gastric inflammation.

EFFECTS OF CRANBERRY POWDER ON BIOMARKERS OF OXIDATIVE STRESS AND GLUCOSE CONTROL IN DB/DB MICE

From: http://www.ncbi.nlm.nih.gov/pubmed/24353827

ABSTRACT

Increased oxidative stress in obese diabetes may have causal effects on diabetic complications, including dyslipidemia. Lipopolysccharides (LPS) along with an atherogenic diet have been found to increase oxidative stress and insulin resistance. Cranberry has been recognized as having beneficial effects on diseases related to oxidative stress. Therefore, we employed obese diabetic animals treated with an atherogenic diet and LPS, with the aim of examining the effects of cranberry powder (CP) on diabetic related metabolic conditions, including lipid profiles, serum insulin and glucose, and biomarkers of oxidative stress. Forty C57BL/KsJ-db/db mice were divided into the following five groups: normal diet + saline, atherogenic diet + saline, atherogenic diet + LPS, atherogenic diet + 5% CP + LPS, and atherogenic diet + 10% CP + LPS. Consumption of an atherogenic diet resulted in elevation of serum total cholesterol and atherogenic index (AI) and reduction of high density lipoprotein (HDL)-cholesterol. However, with 10% CP, the increase in mean HDL-cholesterol level was close to that of the group with a normal diet, whereas AI was maintained at a higher level than that of the group with a normal diet. LPS induced elevated serum insulin level was lowered by greater than 60% with CP (P < 0.05), and mean serum glucose level was reduced by approximately 19% with 5% CP (P > 0.05). Mean activity of liver cytosolic glutathione peroxidase was significantly increased by LPS injection, however it was reduced back to the value without LPS when the diet was fortified with 10% CP (P < 0.05). In groups with CP, a reduction in mean levels of serum protein carbonyl tended to occur in a dose dependent manner. Particularly with 10% CP, a reduction of approximately 89% was observed (P > 0.05). Overall results suggest that fortification of the atherogenic diet with CP may have potential health benefits for obese diabetes with high oxidative stress, by modulation of physical conditions, including some biomarkers of oxidative stress.

SUMMARY

Cranberry extract is full of polyphenols, anthocyanins, antioxidants, and Nrf2 activators that help to make Ultimate Protector+ such an outstanding nutritional supplement.