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Description and Comparison of ORAC Tests for Well Known Plant Ingredients and Ultimate Protector™

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Hank Liers ORAC testsThe term “ORAC” (standing for “Oxygen Radical Absorption Capacity”) describes a test developed by Brunswick Labs as an analytical tool for estimating the antioxidant capacity of substances. The measurement of ORAC value involves an in vitro test of the capacity of a substance to trap peroxyl radicals. Brunswick Labs remains a leader in the development of ORAC tests.

The development of the ORAC test was an important advancement in commercially available analysis of the capacity of various foods and ingredients to trap the peroxyl radical. It has become a de facto standard in the natural products industry. However, the original ORAC method was considered to be just a starting point for comprehensive antioxidant analysis.

The arrival of more comprehensive ORAC tests like Brunswick Labs’ ORAC5.0™ test (also known as ORAC-FN) measuring the antioxidant capacities of five different types of oxidants allows more accurately determination of the total antioxidant quenching power of substances. This helps to better assess the effectiveness of existing antioxidant formulas, and supports development of improved formulas that provide a broad range of antioxidant foods and nutrients that combat all major types of free radicals.

Ultimate Protector - nrf2 activator

FIVE TYPES OF FREE RADICALS AND OXIDANTS

Peroxyl, Hydroxyl, Peroxynitrite, Superoxide Anion, Singlet Oxygen

The fact is that there are a variety of “free radicals” that operate in humans — the most important of which are the primary radicals peroxyl, hydroxy peroxynitrite, superoxide anion, and singlet oxygen. Brunswick Labs has reported that even though the peroxyl radical is the major free radical in the body, it represents no more than 27% of the total antioxidant potential of selected fruits and vegetables. In addition, the original ORAC method favors certain antioxidant substances over others (e.g., anthocyanins over carotenoids) due to the use of a single free radical source (i.e., the peroxyl radical).

Various free radicals are formed, behave, and are defended against differently. They all contribute to 1) a general condition called “oxidative stress,” or cellular damage and 2) broad human health concerns caused, for example, by inflammation, DNA and protein damage. They are each implicated in different health problems — from cardiovascular disease to macular degeneration and Alzheimer’s disease and to skin damage and aging. We provide a brief summary of these free radicals (below).

The Peroxyl Radical is very important in many biological systems, including lipid peroxidation, DNA cleavage, and protein backbone modification. Hydroxyl is highly reactive and cannot be eliminated by our endogenous enzymes (such as SOD and glutathione). It can damage virtually all types of macromolecules: carbohydrates, nucleic acids, lipids, and amino acids. In the skin, hydroxyl radicals are created by UV exposure. Peroxynitrite is a reactive nitrogen species that is particularly harmful to proteins. It has been implicated in the development of certain cancers, hepatitis, and chronic inflammation. In the skin, peroxynitrite contributes to the breakdown of vital proteins, such as collagen.

Superoxide Anion is a precursor of all other reactive oxygen species and sometimes is referred to as “the mother of free radicals.” It is highly toxic and contributes to lipid and DNA damage. Antioxidants that scavenge superoxide anion also help prevent the formation of radicals such as hydrogen peroxide and hydroxyl. Superoxide anion has been linked to hypertension and cardiovascular damage. Singlet Oxygen is generated in the skin by by UV. In vivo, it is linked to the oxidation of LDL cholesterol and cardiovascular disease. Singlet oxygen is highly unstable and durable. Carotenoids are very effective at scavenging singlet oxygen.

The importance of an ORAC test that measures the effectiveness of antioxidant foods, phytochemicals (e.g., polyphenols), vitamins, and other food-based substances against a broader range of free radicals (i.e., beyond the peroxyl radical) becomes apparent when considering how these antioxidants operate in the body. The body faces a diverse group of oxidants, so any test that can provide an estimate of how well a given set of antioxidants will protect cells in reality will also provide a distinct advantage for designing superior antioxidant formulas.

A NEW, MORE COMPREHENSIVE ORAC TEST: ORAC5.0™

Brunswick Labs recently introduced a new, more comprehensive form of ORAC test called ORAC5.0™ (also known as ORAC-FN). This test expands the ORAC platform to measure the antioxidant capacity against each of the five primary reactive oxygen species mentioned above. ORAC5.0™ substantially improves broad-spectrum antioxidant analysis and gives evidence of the diverse antioxidant potential of natural products against radicals other than just the peroxyl radical. Brunswick Labs’ research shows that the antioxidants found in a wide range of natural products are effective against these primary radicals, and that in many cases a preponderance of a product’s antioxidant capacity is described by performance against the four new radicals added to the new ORAC5.0™ panel (which is now the king of ORAC tests).

Table 1.0 (below) shows ORAC5.0™ test results for a range of natural substances known to have significant health benefits and that are broadly used in nutritional supplements. In general, these substances are included at mg levels in supplement formulas. For example, maximum per serving amounts are about 20 mg for astaxanthin, 400 mg for bilberry 25%, 100 mg for grape seed/fruit, 200 mg for green tea extract, 200 mg for coffee berry extract, 100 mg for pine bark, and 100 mg for resveratrol. Considering this the number of total ORAC5.0™ units consumed per serving would typically be in the range of 1,000 to 30,000. Also, it is important to observe that with the exception of green tea extracts none of these ingredients gives excellent protection from the peroxynitrite radical and even green tea extract at the level of 200 mg daily only gives a peroxynitrite radical ORAC value of 400 units.

ULTIMATE PROTECTOR™ DEMONSTRATES HIGH ORAC5.0™ VALUE

In the table, we have also included the results for HPDI’s new ULTIMATE PROTECTOR™ cell protection formula. It can be seen that ULTIMATE PROTECTOR™ has a total ORAC5.0™ value that is more than 50% greater than green tea extract and many times greater than most of the other ingredients. Table 2.0 shows the total ORAC5.0™ units per daily serving. Because the daily recommended serving for ULTIMATE PROTECTOR™ is 2.8 grams (six small capsules), the total daily ORAC5.0™ measures 486,000 units! This value is over 10 times greater than you receive from typical serving sizes of the ingredients shown in the table!! In addition, ULTIMATE PROTECTOR™ provides excellent protection against all five of the free radical types in the body — something none of the specific ingredients do well!!

Table 1.0 ORAC5.0™ Comparative Results

Ingredient Peroxyl Radical Hydroxyl Radical Peroxy-nitrite Radical Super-
oxide Radical
Singlet O2 Radical Total ORAC5.0
Astaxanthin - - - 1,000 9,000 10,000
Bilberry 25% 7,000 25,000 1,000 16,000 5,000 54,000
Cocoa 10,000 28,000 1,000 11,000 2,000 52,000
Grape Seed/Fruit 17,000 47,000 1,000 25,000 4,000 94,000
Green Tea 11,000 41,000 2,000 56,000 3,000 113,000
Coffee Berry 5,000 29,000 1,000 1,000 2,000 38,000
Mangosteen 4,000 8,000 1,000 18,000 4,000 35,000
Pine Bark 7,000 23,000 1,000 17,000 2,000 50,000
Resveratrol 12,000 50,000 1,000 8,000 22,000 93,000
ULTIMATE PROTECTOR 6,300 5,900 2,500 106,000 52,000 173,000
Results are expressed in micro mole TE/g

Table 2.0 ULTIMATE PROTECTOR™ ORAC Units Per Serving

ORAC5.0 Units Per Serving* Free Radical Type
17,700 Peroxyl Radical is very important in many biological systems, including lipid peroxidation, DNA cleavage, and protein backbone modification.
16,500 Hydroxyl is highly reactive and cannot be eliminated by our endogenous enzymes. It damages virtually all types of macromolecules: carbohydrates, nucleic acids, lipids, and amino acids. In the skin, hydroxyl radicals are created by UV exposure.
6,900 Peroxynitrite is a reactive nitrogen species that is particularly harmful to proteins. It has been implicated in the development of certain cancers, hepatitis, and chronic inflammation. In the skin, peroxynitrite contributes to the breakdown of vital proteins, such
as collagen.
146,000 Singlet Oxygen is generated in the skin by UV exposure. It is linked to the oxidation of LDL cholesterol and cardiovascular disease.
299,000 Superoxide Anion is a precursor of all other reactive oxygen species – sometimes referred to as “the mother of free radicals.” It is highly toxic and contributes to lipid and DNA damage.
486,000 Total Per Daily Serving of Six Capsules (2.81g)

Review the Brunswick Labs Ultimate Protector™ ORAC5.0™ Test Report Here

ADDITIONAL RESOURCES

 Questions & Answers about Ultimate Protector

The Amazing Healing Potential of Natural Nrf2 Activators

Ultimate Protector™ and the Role of Foundational Supplements for Health

New Directions For Preventing Free-Radical Damage

Natural Phytochemical Nrf2 Activators for Chemoprevention

Ultimate Protector™ Brunswick Labs ORAC5.0™ Test Results

Description and Comparison of ORAC Tests for Well Known Plant Ingredients and Ultimate Protector™

Ultimate Protector™: First Impressions

Ultimate Protector™ ORAC5.0™ Test Results

Preventing Free-Radical Damage Using Ultimate Protector™

New Directions for Preventing Free-Radical Damage

Natural Phytochemical Nrf2 Activators for Chemoprevention

Ultimate Protector flyer (.pdf)

Hank Liers, PhD

Hank Liers, PhD

Dr. Hank Liers is the CEO and chief product formulator for Health Products Distributors, Inc. He has been studying and using natural means of achieving health since 1984. Dr. Liers received his PhD in physics in 1969 from the University of Minnesota and has applied his analytical abilities to learning and applying a scientific approach to nutrition.